Cat.No. : CSC-RO0215 Host Cell: CHO-K1
| Cat. No. | CSC-RO0215 |
| Description | This cell line is engineered to stably overexpress the monkey TIGIT in CHO-K1 cells. |
| Introduction | This cell line is constructed by transfection of monkey T cell immunoreceptor with Ig and ITIM domains (TIGIT) into CHO-K1, followed by stable cell selection. The expression of TIGIT has been analyzed through flow cytometry. |
| Gene | TIGIT |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Monkey TIGIT Stable Cell Line - CHO-K1 is engineered to express the TIGIT (T cell immunoreceptor with Ig and ITIM domains) protein, a key immune checkpoint receptor. This cell line is significant as it allows researchers to study the interaction of TIGIT with its ligands and the downstream signaling pathways, which are crucial for understanding the immune checkpoint blockade strategies in cancer therapy.
A: By providing a stable platform for TIGIT expression, this cell line enables researchers to screen for potential drugs that can modulate TIGIT activity. This is vital for the development of novel cancer immunotherapies that aim to enhance the immune system's ability to recognize and eliminate cancer cells.
A: Ethical considerations include ensuring that the cell line is sourced from reputable suppliers, that the genetic modifications do not cause undue harm to the cells, and that the research adheres to guidelines for the use of animal-derived materials. Additionally, the use of such cell lines should be justified by the potential scientific benefits and the absence of viable alternatives.
A: Regulatory requirements vary by country but generally include ensuring the cell line's quality, safety, and efficacy, as well as compliance with Good Manufacturing Practices (GMP) for any clinical applications. The cell line must be thoroughly characterized, and any genetic modifications must be well-documented and validated.
A: The cell line is typically cryopreserved in liquid nitrogen using a freezing medium containing dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS). For revival, cells are thawed rapidly and transferred to a culture medium for recovery and expansion.
A: Researchers should follow standard cell culture practices, including aseptic techniques, to prevent contamination. They should also be aware of the potential for genetic drift and regularly verify the stability of the TIGIT expression to ensure the reliability of experimental results.
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