Monkey TIGIT Stable Cell Line - CHO-K1

Name: Monkey TIGIT Stable Cell Line - CHO-K1
SKU: CSC-RO0215
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0215

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0215
Description	This cell line is engineered to stably overexpress the monkey TIGIT in CHO-K1 cells.
Introduction	This cell line is constructed by transfection of monkey T cell immunoreceptor with Ig and ITIM domains (TIGIT) into CHO-K1, followed by stable cell selection. The expression of TIGIT has been analyzed through flow cytometry.
Target Gene	TIGIT
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the significance of the Monkey TIGIT Stable Cell Line - CHO-K1 in the study of immune checkpoint inhibitors?

A: The Monkey TIGIT Stable Cell Line - CHO-K1 is engineered to express the TIGIT (T cell immunoreceptor with Ig and ITIM domains) protein, a key immune checkpoint receptor. This cell line is significant as it allows researchers to study the interaction of TIGIT with its ligands and the downstream signaling pathways, which are crucial for understanding the immune checkpoint blockade strategies in cancer therapy.

How does the Monkey TIGIT Stable Cell Line - CHO-K1 contribute to the development of cancer immunotherapies?

A: By providing a stable platform for TIGIT expression, this cell line enables researchers to screen for potential drugs that can modulate TIGIT activity. This is vital for the development of novel cancer immunotherapies that aim to enhance the immune system's ability to recognize and eliminate cancer cells.

What are the ethical considerations for using the Monkey TIGIT Stable Cell Line - CHO-K1 in research?

A: Ethical considerations include ensuring that the cell line is sourced from reputable suppliers, that the genetic modifications do not cause undue harm to the cells, and that the research adheres to guidelines for the use of animal-derived materials. Additionally, the use of such cell lines should be justified by the potential scientific benefits and the absence of viable alternatives.

What are the regulatory requirements for the use of the Monkey TIGIT Stable Cell Line - CHO-K1 in clinical trials?

A: Regulatory requirements vary by country but generally include ensuring the cell line's quality, safety, and efficacy, as well as compliance with Good Manufacturing Practices (GMP) for any clinical applications. The cell line must be thoroughly characterized, and any genetic modifications must be well-documented and validated.

How is the Monkey TIGIT Stable Cell Line - CHO-K1 preserved and revived for future use?

A: The cell line is typically cryopreserved in liquid nitrogen using a freezing medium containing dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS). For revival, cells are thawed rapidly and transferred to a culture medium for recovery and expansion.

What are the precautions researchers should take when working with the Monkey TIGIT Stable Cell Line - CHO-K1?

A: Researchers should follow standard cell culture practices, including aseptic techniques, to prevent contamination. They should also be aware of the potential for genetic drift and regularly verify the stability of the TIGIT expression to ensure the reliability of experimental results.

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