Mouse Tigit Stable Cell Line-CHO-K1

Name: Mouse Tigit Stable Cell Line-CHO-K1
SKU: CSC-RO0041
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0041

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0041
Description	This cell line is a stably transfected cell line which expresses Mouse Tigit without any tag.
Target Gene	Tigit
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tigit T cell immunoreceptor with Ig and ITIM domains [ Mus musculus ]
Gene Symbol	Tigit
Synonyms	Vstm3; ENSMUSG00000071552
GeneID	100043314
mRNA Refseq	NM_001146325.1
Protein Refseq	NP_001139797.1
Chromosome Location	16 B4; 16
Function	receptor binding; receptor binding;

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TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells (NK). It is also identified as WUCAM and Vstm3. TIGIT binds to CD155 (PVR) on dendritic cells (DCs), macrophages, etc. with high affinity, and also to CD112 (PVRL2) with lower affinity. Research has shown that TIGIT-Fc fusion proteins interact with PVR on dendritic cells and increase IL-10 secretion, while decreasing IL-12 secretion under LPS stimulation. TIGIT-Fc fusion proteins also inhibit T cell activation in vivo. TIGIT's inhibition of NK cytotoxicity can be blocked by antibodies against its interaction with PVR, which is directed through its ITIM domain.

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