Cat.No. : CSC-RO1046 Host Cell: HEK293T
| Cat. No. | CSC-RO1046 |
| Description | HEK293-Cldn18-A2 cell line is engineered to stably overexpress mouse Cldn18 isoform 2 (Cldn18.2). |
| Gene | Cldn18-A2 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: When using Mouse Cldn18-A2 Stable Cell Line-HEK293 in research involving human tissues or clinical applications, researchers must adhere to ethical guidelines, obtain appropriate informed consent when working with human samples, and ensure compliance with institutional review board (IRB) or ethics committee requirements. Additionally, ethical considerations related to genetic manipulation and potential future therapeutic applications should be carefully evaluated.
A: The interactions between Mouse Cldn18-A2 and other proteins or molecules are actively studied areas of research. Understanding these interactions may have implications for drug development, particularly in the context of tight junction modulators or therapies targeting diseases associated with altered epithelial barrier function. Research into potential therapeutic applications is ongoing.
A: Variations in Mouse Cldn18-A2 expression levels in Mouse Cldn18-A2 Stable Cell Line-HEK293 can significantly impact experimental outcomes, especially in studies where precise control of protein expression is required. Researchers can modulate Mouse Cldn18-A2 expression using inducible systems, such as tetracycline-regulated promoters, or by using CRISPR-Cas9 technology to knock down or overexpress the gene as needed for their experiments. These strategies allow for more controlled experimentation with the cell line.
A: One potential limitation is that Mouse Cldn18-A2 Stable Cell Line-HEK293 is a simplified model of epithelial biology, and its behavior may not fully represent the complexity of in vivo systems. Additionally, researchers should consider that the stable expression of a single protein may not fully recapitulate the natural context of tight junctions in a tissue or organ. Careful validation and comparison with other models may be necessary.
A: The Mouse Cldn18-A2 Stable Cell Line-HEK293 has allowed researchers to investigate the specific functions and interactions of claudin-18-A2, which is not as extensively studied as other tight junction proteins. This cell line has provided insights into the role of claudin-18-A2 in regulating paracellular transport and epithelial permeability, which may have implications for understanding diseases related to tight junction dysfunction.
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