Cat.No. : CSC-RO0289 Host Cell: HeLa
| Cat. No. | CSC-RO0289 |
| Description | This cell line is constructed by transduction of human angiotensin I converting enzyme 2 (ACE2) into HeLa cells, followed by stable cell selection. HeLa is derived from cervical cancer cells and is a commonly used human cell line in scientific research. HeLa-human ACE2 cell line can be used for in vitro screening and characterization of drug candidates against SARS-CoV, SARS-Cov2 (2019-nCoV). |
| Gene | ACE2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HeLa |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Study the mechanisms of viral entry and replication 2. Research and in vitro screening of potential drugs 3. Vaccine development 4. The production of pseudotyped viruses |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The SARS-CoV-2 virus binds to ACE2, a cell surface receptor. Since stopping the spread of SARS-CoV-2 is a top priority during the COVID-19 pandemic, we report here the S-nitrosylation of several proteins involved in the SARS-CoV-2 infection, including the receptor for viral entry, angiotensin-converting enzyme 2 (ACE2). This reaction inhibits the binding of ACE2 to the SARS-CoV-2 Spike protein, which in turn inhibits viral entry, infectivity, and cytotoxicity. Additionally, amino adamantane compounds block coronavirus ion channels formed by envelope (E) protein. In light of this, they created dual-mechanism amino adamantane nitrate compounds, which are non-toxic and active in vitro and in vivo in the Syrian hamster COVID-19 model. These compounds inhibit viral entry and thereby spread infection by S-nitrosylating ACE2 via targeted delivery of the drug after E-protein channel blockade.
Figure 1. When evaluating SNOC's effects on cell lines, the researchers found that it might raise ACE2's S-nitrosylation and prevent it from binding to the SARS-CoV-2 Spike protein. The biotin-switch assay was used to examine the cell lysates of HeLa-ACE2 cells that had been treated with SNOC. Furthermore, upon incubation with recombinant SARS-CoV-2 Spike protein, HeLa and HeLa-ACE2 cells exposed to SNOC demonstrated decreased binding of Spike protein, as demonstrated by a fluorescence intensity experiment. (Oh CK, et al., 2023)
A: The cell line stably expresses the ACE2 gene, which is a key component of the RAAS. By studying the function of ACE2 in these cells, researchers can better understand its role in regulating blood pressure and fluid balance, which is crucial for understanding the pathogenesis of cardiovascular diseases.
A: The cell line can be utilized to investigate the interaction between the SARS-CoV-2 virus and its human host receptor, ACE2. By examining this interaction, researchers can identify potential points of intervention to block viral entry, leading to the development of new antiviral strategies.
A: The cell line can be used to study the diverse physiological roles of ACE2, including its involvement in the immune response and the modulation of inflammation. This can provide insights into how ACE2 influences immune cell function and contributes to the regulation of inflammatory processes.
A: By providing a model system for studying the effects of modulating ACE2 expression, the cell line can aid in the development of gene therapies that target the ACE2 gene. This can help researchers test the efficacy and safety of such therapies for treating conditions related to the RAAS.
A: The cell line can be used to model different ACE2 polymorphisms and investigate their impact on cellular function and disease susceptibility. This can help researchers understand how genetic variations in the ACE2 gene influence individual responses to diseases and potential therapeutic outcomes.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Human ACE2 Stable Cell Line - HeLa is engineered to ensure high levels of ACE2 expression. This is crucial for research studies focusing on the interaction between human cells and the novel coronavirus, as ACE2 is the main receptor for the virus entry into cells. High expression levels provide a consistent and reliable model for studying virus-host interactions and assessing potential therapies.
Consistency is key in scientific research, and the Human ACE2 Stable Cell Line - HeLa offers reproducible results due to its genetic stability. This stable expression ensures that experiments can be repeated with the same expected outcomes, which is essential for validating studies and developing therapeutics.
This cell line comes in a ready-to-use format, significantly reducing preparation time for us. The Human ACE2 Stable Cell Line - HeLa eliminates the need for lengthy cell culture setup procedures, allowing for immediate commencement of experimental protocols, which accelerates the research process.
The Human ACE2 Stable Cell Line - HeLa can be used across a wide range of biomedical applications, including virology, immunology, and toxicology. This versatility makes it an invaluable tool for us looking to explore ACE2's role in different scientific contexts, enhancing its utility in diverse research settings.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
