Cat.No. : CSC-RO0641 Host Cell: HEK293T
| Cat. No. | CSC-RO0641 |
| Description | This cell line is engineered to stably overexpressing human angiotensin I converting enzyme 2 (ACE2). HEK293T is derived from HEK293 and is commonly used in scientific research. HEK293T-human ACE2 cell line can be used for in vitro screening and characterization of drug candidates against SARS-CoV, SARS-Cov2 (2019-nCoV). |
| Gene | ACE2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Study the mechanisms of viral entry and replication 2. Research and in vitro screening of potential drugs 3. Vaccine development 4. The production of pseudotyped viruses |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Previously, phthalocyanine and hypericin were recognized as potential inhibitors of SARS-CoV-2 Spike glycoprotein fusion via a virtual screening process. Researchers demonstrated the potential of ACE2 Stable Cell Lines in investigating the inhibitory effects of phthalocyanine and hypericin on SARS-CoV-2 entry. Computational simulations revealed their multi-target inhibitory potential by binding to key protein regions and inserting into the viral membrane. Pre-treatment of pseudovirus expressing SARS-CoV-2 Spike protein with low compound concentrations inhibited viral entry into cells, suggesting direct targeting of the viral envelope surface. This computational and in vitro evidence supports the role of hypericin and phthalocyanine as promising SARS-CoV-2 entry inhibitors, aligning with literature on their efficacy in treating COVID-19 patients.
Figure 1. The effects of HEK293T-ACE2 cells on ROS levels and SARS-CoV-2 pseudovirus infection were assessed by researchers. The HEK293T/ACE2 (CSC-RO0641/Creative Biogene) cell lines are sourced from Creative Biogene. (Romeo, A., et al., 2023)
A: HEK293T cells were likely chosen for their high transfection efficiency and robust expression system, essential for studying ACE2 expression, viral entry, and its role in physiological and pathological processes.
A: Stability was likely confirmed through methods such as immunoblotting, flow cytometry, or functional assays assessing ACE2-mediated viral entry or signaling, with continuous selection pressure applied.
A: Characterization may involve analysis of ACE2 localization, binding kinetics to viral spike proteins, downstream signaling pathways, and functional implications in viral infection, particularly with coronaviruses.
A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with primary human cells or relevant animal models helps validate the relevance of ACE2 expression in viral tropism, pathogenesis, and therapeutic interventions, guiding antiviral drug development and vaccine strategies.
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Exceptional quality! The Human ACE2 Stable Cell Line in HEK293T cells has provided unparalleled reliability and consistency in my studies of viral entry mechanisms. Its stable expression of ACE2 has been crucial in elucidating the pathogenesis of viral infections.
Reliable and robust! The stable expression of ACE2 in HEK293T cells has enabled precise and accurate measurements of viral entry kinetics. It has been instrumental in dissecting the molecular interactions between viruses and host cells.
Simplifying complex research! With the Human ACE2 Stable Cell Line, I've been able to investigate viral tropism and host cell specificity with confidence. Its stable expression has streamlined my experiments, allowing for more insightful data analysis.
Impressive performance! The Human ACE2 Stable Cell Line has consistently delivered reproducible results, exceeding my expectations. Its robust ACE2 expression has facilitated in-depth studies of virus-host interactions and potential therapeutic interventions.
A game-changer in viral research! The Human ACE2 Stable Cell Line has revolutionized my approach to studying viral infections. Its stable expression of ACE2 has provided a solid foundation for investigating the pathophysiology of infectious diseases and developing antiviral strategies.
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