Human ACE2 Stable Cell Line - HEK293T

Name: Human ACE2 Stable Cell Line - HEK293T
SKU: CSC-RO0641
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0641

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Cat. No.	CSC-RO0641
Description	This cell line is engineered to stably overexpressing human angiotensin I converting enzyme 2 (ACE2). HEK293T is derived from HEK293 and is commonly used in scientific research. HEK293T-human ACE2 cell line can be used for in vitro screening and characterization of drug candidates against SARS-CoV, SARS-Cov2 (2019-nCoV).
Target Gene	ACE2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Study the mechanisms of viral entry and replication 2. Research and in vitro screening of potential drugs 3. Vaccine development 4. The production of pseudotyped viruses
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]
Gene Symbol	ACE2
Synonyms	ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; ACEH;
Gene ID	59272
Uni Prot ID	Q9BYF1
m RNA Refseq	BC039902
Chromosome Location	Xp22
Function	carboxypeptidase activity; endopeptidase activity; glycoprotein binding; metal ion binding; NOT metallopeptidase activity; peptidase activity; NOT peptidyl-dipeptidase activity; viral receptor activity; zinc ion binding;
Pathway	ACE Inhibitor Pathway, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem; Renin-angiotensin system, organism-specific biosystem; Renin-angiotensin system, conserved biosystem;
MIM	300335

CSC-RO0641-Human ACE2 Stable Cell Line - HEK293T-Datasheet

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Background

Case Study

Applications

Publications

Q & A

Customer Reviews

ACE2, a key component of the renin-angiotensin system (RAS), was first identified and characterized in 2000. It plays a vital role in regulating cardiovascular and renal functions. Subsequent advancements in cell line engineering, particularly in recombinant DNA technology, facilitated the development of stable cell lines expressing human ACE2 (hACE2). Notably, the HEK293T cell line, derived from human embryonic kidney cells, emerged as a preferred choice due to its robust growth characteristics and transfection efficiency. Establishment of HEK293T stable cell lines expressing hACE2 has significantly contributed to studies investigating the interaction between ACE2 and various pathogens, particularly coronaviruses. This model has been instrumental in understanding viral entry mechanisms and identifying potential therapeutic targets.

Previously, phthalocyanine and hypericin were recognized as potential inhibitors of SARS-CoV-2 Spike glycoprotein fusion via a virtual screening process. Researchers demonstrated the potential of ACE2 Stable Cell Lines in investigating the inhibitory effects of phthalocyanine and hypericin on SARS-CoV-2 entry. Computational simulations revealed their multi-target inhibitory potential by binding to key protein regions and inserting into the viral membrane. Pre-treatment of pseudovirus expressing SARS-CoV-2 Spike protein with low compound concentrations inhibited viral entry into cells, suggesting direct targeting of the viral envelope surface. This computational and in vitro evidence supports the role of hypericin and phthalocyanine as promising SARS-CoV-2 entry inhibitors, aligning with literature on their efficacy in treating COVID-19 patients.

Figure 1. The effects of HEK293T-ACE2 cells on ROS levels and SARS-CoV-2 pseudovirus infection were assessed by researchers. The HEK293T/ACE2 (CSC-RO0641/Creative Biogene) cell lines are sourced from Creative Biogene. (Romeo, A., et al., 2023)

1. Virus Research: Utilize HEK293T cells expressing Human ACE2 for studying viral entry mechanisms, exemplified by investigating SARS-CoV-2 infectivity and assessing potential therapeutic interventions.

2. Drug Screening: Employ HEK293T Human ACE2 Stable Cell Line to screen candidate drugs targeting viral infections, facilitating the development of antiviral agents against coronaviruses and other pathogens.

3. Vaccine Development: Utilize HEK293T cells expressing Human ACE2 to evaluate vaccine efficacy by assessing neutralizing antibody production and vaccine-induced immune responses against SARS-CoV-2 and related viruses.

4. Pathogenesis Studies: Investigate host-pathogen interactions by infecting HEK293T Human ACE2 Stable Cell Line with coronaviruses, elucidating viral replication dynamics, and exploring host factors influencing infection outcomes.

5. Protein Interaction Studies: Utilize HEK293T Human ACE2 Stable Cell Line to study protein-protein interactions involved in viral entry, such as Spike protein binding, facilitating the design of therapeutics targeting viral attachment and entry mechanisms.

Publications

Romeo A, Cappelli G, Iacovelli F, et al. Computational and experimental validation of phthalocyanine and hypericin as effective SARS-CoV-2 fusion inhibitors[J]. Journal of Biomolecular Structure and Dynamics, 2023: 1-15.

Customer Q&As

What factors influenced the choice of HEK293T cells for establishing the stable ACE2 cell line?

A: HEK293T cells were likely chosen for their high transfection efficiency and robust expression system, essential for studying ACE2 expression, viral entry, and its role in physiological and pathological processes.

How was the stability of ACE2 expression verified and maintained in this HEK293T stable cell line?

A: Stability was likely confirmed through methods such as immunoblotting, flow cytometry, or functional assays assessing ACE2-mediated viral entry or signaling, with continuous selection pressure applied.

Can you elaborate on the characterization of ACE2 expression in the HEK293T stable cell line, including its subcellular localization and interaction with viral ligands?

A: Characterization may involve analysis of ACE2 localization, binding kinetics to viral spike proteins, downstream signaling pathways, and functional implications in viral infection, particularly with coronaviruses.

What quality control measures were employed during the generation of this stable cell line?

A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.

How does the expression pattern and functional properties of ACE2 in this stable cell line relate to its physiological roles and relevance in viral infection, particularly in the context of emerging pathogens like SARS-CoV-2?

A: Comparative analysis with primary human cells or relevant animal models helps validate the relevance of ACE2 expression in viral tropism, pathogenesis, and therapeutic interventions, guiding antiviral drug development and vaccine strategies.

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Customer Reviews

Crucial reliability

Exceptional quality! The Human ACE2 Stable Cell Line in HEK293T cells has provided unparalleled reliability and consistency in my studies of viral entry mechanisms. Its stable expression of ACE2 has been crucial in elucidating the pathogenesis of viral infections.

United States

2022-02-25

Enabling precise measurements

Reliable and robust! The stable expression of ACE2 in HEK293T cells has enabled precise and accurate measurements of viral entry kinetics. It has been instrumental in dissecting the molecular interactions between viruses and host cells.

Germany

2020-12-31

Confidence in research

Simplifying complex research! With the Human ACE2 Stable Cell Line, I've been able to investigate viral tropism and host cell specificity with confidence. Its stable expression has streamlined my experiments, allowing for more insightful data analysis.

Germany

2023-06-10

Reliable

Impressive performance! The Human ACE2 Stable Cell Line has consistently delivered reproducible results, exceeding my expectations. Its robust ACE2 expression has facilitated in-depth studies of virus-host interactions and potential therapeutic interventions.

United Kingdom

2020-06-12

Revolutionizing research

A game-changer in viral research! The Human ACE2 Stable Cell Line has revolutionized my approach to studying viral infections. Its stable expression of ACE2 has provided a solid foundation for investigating the pathophysiology of infectious diseases and developing antiviral strategies.

Germany

2022-12-23

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Human ACE2 Stable Cell Line - HEK293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Background

Case Study

Applications

Publications

Q & A

Customer Reviews

Publications

What factors influenced the choice of HEK293T cells for establishing the stable ACE2 cell line?

How was the stability of ACE2 expression verified and maintained in this HEK293T stable cell line?

Can you elaborate on the characterization of ACE2 expression in the HEK293T stable cell line, including its subcellular localization and interaction with viral ligands?

What quality control measures were employed during the generation of this stable cell line?

How does the expression pattern and functional properties of ACE2 in this stable cell line relate to its physiological roles and relevance in viral infection, particularly in the context of emerging pathogens like SARS-CoV-2?

Crucial reliability

Enabling precise measurements

Confidence in research

Reliable

Revolutionizing research

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