Human ACE2 Stable Cell Line - NIH3T3

Name: Human ACE2 Stable Cell Line - NIH3T3
SKU: CSC-RO0291
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0291

Host Cell : NIH3T3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0291
Description	This cell line is generated by transduction of human angiotensin I converting enzyme 2 (ACE2) into NIH3T3 cells, followed by stable cell selection. NIH3T3 cell line is derived from mouse embryonic fibroblasts. NIH3T3-human ACE2 cell line is an ideal cell model for in vitro screening and characterization of potential drugs against SARS-CoV, SARS-Cov2 (2019-nCoV).
Target Gene	ACE2
Gene Species	Homo sapiens (Human)
Host Cell	NIH3T3
Host Cell Species	Mus musculus (Mouse)
Applications	1. Study the mechanisms of viral entry and replication 2. Research and in vitro screening of potential drugs 3. Vaccine development 4. The production of pseudotyped viruses
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]
Gene Symbol	ACE2
Synonyms	ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; ACEH;
Gene ID	59272
Uni Prot ID	Q9BYF1
m RNA Refseq	BC039902
Chromosome Location	Xp22
Function	carboxypeptidase activity; endopeptidase activity; glycoprotein binding; metal ion binding; NOT metallopeptidase activity; peptidase activity; NOT peptidyl-dipeptidase activity; viral receptor activity; zinc ion binding;
Pathway	ACE Inhibitor Pathway, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem; Renin-angiotensin system, organism-specific biosystem; Renin-angiotensin system, conserved biosystem;
MIM	300335

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