Cat.No. : CSC-RO0292 Host Cell: HEK293
| Cat. No. | CSC-RO0292 |
| Description | This cell line is engineered to stably overexpressing human angiotensin I converting enzyme 2 (ACE2). HEK 293 is a human embryonic kidney cell line which has been widely used in cell biology research. HEK293-human ACE2 cell line is a powerful tool for in vitro screening and characterization of potential drugs against SARS-CoV, SARS-Cov2 (2019-nCoV). |
| Gene | ACE2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Study the mechanisms of viral entry and replication 2. Research and in vitro screening of potential drugs 3. Vaccine development 4. The production of pseudotyped viruses |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The significance of cell lines in the fight against SARS-CoV-2 is emphasized by the researchers, who use circulating extracellular vesicles (EVs) expressing ACE2 (evACE2) to compete with cellular ACE2 and neutralize virus entry. evACE2 is 135 times more potent than vesicle-free rhACE2 in blocking viral spike protein RBD binding, and it is 60 to 80 times more effective against both pseudotyped and authentic SARS-CoV-2. Additionally, evACE2 shields hACE2 transgenic mice from severe lung damage and death caused by SARS-CoV-2, and its capacity to inhibit infections by multiple SARS-CoV-2 variants (α, β, δ) highlights its potential as a broad-spectrum therapeutic against present-day coronaviruses that use the ACE2 receptor.
Figure 1. The researchers demonstrated that ACE2 Stable Cell Line - HEK293 cells effectively neutralize RBD-binding and SARS-CoV-2 variant infections. Using flow profiles, they assessed ACE2+ EVs' (evACE2) inhibition of viral binding, showing significant neutralization compared to ACE2- EVs (evCon). IC50 analysis confirmed evACE2's potency in inhibiting both wild-type and variant SARS-CoV-2 infections. (El-Shennawy L, et al., 2022)
A: The Human ACE2 Stable Cell Line - HEK293 was created by stably expressing the human Angiotensin-Converting Enzyme 2 (ACE2) protein in Human Embryonic Kidney 293 (HEK293) cells. This was done to meet the research demand for understanding how the SARS-CoV-2 virus enters human cells via the ACE2 receptor. By utilizing this cell line, researchers can better understand the mechanism of viral invasion, thereby developing effective antiviral strategies and medications.
A: The Human ACE2 Stable Cell Line - HEK293 demonstrates its unique value in virology research, drug screening, and studying the role of ACE2 in human health and disease. It becomes a valuable tool especially in studying how SARS-CoV-2 and its variants interact with host cells, as it can be used to assess viral infectivity and the inhibitory effects of drugs.
A: The Human ACE2 Stable Cell Line - HEK293 is significant for future vaccine development as it provides an efficient platform to evaluate the neutralizing capability of vaccine candidates against SARS-CoV-2 and its variants. By testing vaccine-induced antibody responses in this cell line, researchers can screen for effective vaccine candidates, speeding up the vaccine development process.
A: The primary advantage of the Human ACE2 Stable Cell Line - HEK293 in simulating the natural infection process is its ability to efficiently and stably express the ACE2 receptor, which is key for the entry of SARS-CoV-2 into cells. This efficient expression of ACE2 allows the cell line to mimic the virus's natural infection process, providing ideal conditions for studying the mechanism of viral invasion and evaluating antiviral strategies.
A: The key technical challenges in the establishment of the Human ACE2 Stable Cell Line - HEK293 included efficiently integrating the ACE2 gene into the genome of HEK293 cells and ensuring its stable expression without affecting cell growth and function. These challenges were overcome by using efficient transfection techniques, such as viral vector-mediated gene transduction, and screening for cell clones that stably express ACE2.
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Due to stability and high expression levels, you can achieve efficient experimental results, advancing research progress in relevant fields.
High expression of Human ACE2 in HEK293 enables efficient detection and study of its functions.
The Human ACE2 Stable Cell Line - HEK293 provides an experimental platform for studying the interaction between coronaviruses and host cells.
This cell line assists in screening therapeutic drugs targeting ACE2-mediated viral infections.
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