Human ACE2 Stable Cell Line - HEK293

Name: Human ACE2 Stable Cell Line - HEK293
SKU: CSC-RO0292
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0292

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

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Cat. No.	CSC-RO0292
Description	This cell line is engineered to stably overexpressing human angiotensin I converting enzyme 2 (ACE2). HEK 293 is a human embryonic kidney cell line which has been widely used in cell biology research. HEK293-human ACE2 cell line is a powerful tool for in vitro screening and characterization of potential drugs against SARS-CoV, SARS-Cov2 (2019-nCoV).
Target Gene	ACE2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Study the mechanisms of viral entry and replication 2. Research and in vitro screening of potential drugs 3. Vaccine development 4. The production of pseudotyped viruses
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]
Gene Symbol	ACE2
Synonyms	ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; ACEH;
GeneID	59272
Uni ProtID	Q9BYF1
mRNA Refseq	BC039902
Chromosome Location	Xp22
Function	carboxypeptidase activity; endopeptidase activity; glycoprotein binding; metal ion binding; NOT metallopeptidase activity; peptidase activity; NOT peptidyl-dipeptidase activity; viral receptor activity; zinc ion binding;
Pathway	ACE Inhibitor Pathway, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem; Renin-angiotensin system, organism-specific biosystem; Renin-angiotensin system, conserved biosystem;
MIM	300335

CSC-RO0292-Human ACE2 Stable Cell Line - HEK293-Datasheet

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The Human ACE2 (Angiotensin-Converting Enzyme 2) gene encodes a membrane-bound protein that acts as a carboxypeptidase, playing a key role in the regulation of the renin-angiotensin-aldosterone system. ACE2 is involved in the conversion of angiotensin II to angiotensin 1-7, which has vasodilatory effects and is protective against cardiovascular diseases. However, ACE2 is also the receptor for the SARS-CoV-2 virus, making it central to the pathogenesis of COVID-19.

The HEK293 cell line has been modified to stably express the Human ACE2 gene. This stable cell line provides a valuable tool for studying the physiological functions of ACE2 and its role in the pathogenesis of COVID-19. Researchers can use this cell line to investigate the interaction of ACE2 with SARS-CoV-2, its role in viral entry and replication, and the development of potential therapeutic strategies to target the ACE2 pathway.

The significance of cell lines in the fight against SARS-CoV-2 is emphasized by the researchers, who use circulating extracellular vesicles (EVs) expressing ACE2 (evACE2) to compete with cellular ACE2 and neutralize virus entry. evACE2 is 135 times more potent than vesicle-free rhACE2 in blocking viral spike protein RBD binding, and it is 60 to 80 times more effective against both pseudotyped and authentic SARS-CoV-2. Additionally, evACE2 shields hACE2 transgenic mice from severe lung damage and death caused by SARS-CoV-2, and its capacity to inhibit infections by multiple SARS-CoV-2 variants (α, β, δ) highlights its potential as a broad-spectrum therapeutic against present-day coronaviruses that use the ACE2 receptor.

Figure 1 depicts the neutralization effect of the ACE2 stable cell line (HEK293 cell line) in neutralising neo-coronavirus (SARS-CoV-2) RBD binding and its variants in infection. (doi: 10.1038/s41467-021-27893-2) Figure 1. The researchers demonstrated that ACE2 Stable Cell Line - HEK293 cells effectively neutralize RBD-binding and SARS-CoV-2 variant infections. Using flow profiles, they assessed ACE2⁺ EVs' (evACE2) inhibition of viral binding, showing significant neutralization compared to ACE2- EVs (evCon). IC50 analysis confirmed evACE2's potency in inhibiting both wild-type and variant SARS-CoV-2 infections. (El-Shennawy L, et al., 2022)

The Human ACE2 Stable Cell Line - HEK293 is a cell line that expresses the ACE2 gene, which encodes for angiotensin-converting enzyme 2. This enzyme plays a crucial role in the renin-angiotensin-aldosterone system and has been identified as the receptor for SARS-CoV-2, the virus responsible for COVID-19. The applications of this cell line are:

Viral Entry Mechanisms: This cell line is invaluable for studying the interaction between ACE2 and SARS-CoV-2, providing insights into the viral entry process and the development of antiviral strategies.

Drug Screening: Researchers can use the Human ACE2 Stable Cell Line to screen for compounds that inhibit the interaction between ACE2 and SARS-CoV-2, potentially leading to the discovery of new antiviral drugs.

Cardiovascular Research: ACE2 is also involved in the regulation of blood pressure and heart function. This cell line can be used to study the role of ACE2 in cardiovascular diseases and to develop treatments for conditions such as hypertension and heart failure.

Customer Q&As

What is the background of the establishment of the Human ACE2 Stable Cell Line - HEK293? How does this cell line meet the needs of current research fields?

A: The Human ACE2 Stable Cell Line - HEK293 was created by stably expressing the human Angiotensin-Converting Enzyme 2 (ACE2) protein in Human Embryonic Kidney 293 (HEK293) cells. This was done to meet the research demand for understanding how the SARS-CoV-2 virus enters human cells via the ACE2 receptor. By utilizing this cell line, researchers can better understand the mechanism of viral invasion, thereby developing effective antiviral strategies and medications.

In which specific research areas does the Human ACE2 Stable Cell Line - HEK293 demonstrate its unique value?

A: The Human ACE2 Stable Cell Line - HEK293 demonstrates its unique value in virology research, drug screening, and studying the role of ACE2 in human health and disease. It becomes a valuable tool especially in studying how SARS-CoV-2 and its variants interact with host cells, as it can be used to assess viral infectivity and the inhibitory effects of drugs.

What is the significance of the establishment of the Human ACE2 Stable Cell Line - HEK293 for future vaccine development?

A: The Human ACE2 Stable Cell Line - HEK293 is significant for future vaccine development as it provides an efficient platform to evaluate the neutralizing capability of vaccine candidates against SARS-CoV-2 and its variants. By testing vaccine-induced antibody responses in this cell line, researchers can screen for effective vaccine candidates, speeding up the vaccine development process.

What are the advantages of the Human ACE2 Stable Cell Line - HEK293 in simulating the natural infection process?

A: The primary advantage of the Human ACE2 Stable Cell Line - HEK293 in simulating the natural infection process is its ability to efficiently and stably express the ACE2 receptor, which is key for the entry of SARS-CoV-2 into cells. This efficient expression of ACE2 allows the cell line to mimic the virus's natural infection process, providing ideal conditions for studying the mechanism of viral invasion and evaluating antiviral strategies.

What are the key technical challenges in the establishment of the Human ACE2 Stable Cell Line - HEK293, and how were these challenges overcome?

A: The key technical challenges in the establishment of the Human ACE2 Stable Cell Line - HEK293 included efficiently integrating the ACE2 gene into the genome of HEK293 cells and ensuring its stable expression without affecting cell growth and function. These challenges were overcome by using efficient transfection techniques, such as viral vector-mediated gene transduction, and screening for cell clones that stably express ACE2.

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Customer Reviews

Efficient research

Due to stability and high expression levels, you can achieve efficient experimental results, advancing research progress in relevant fields.

United Kingdom

2022-08-11

Efficient detection

High expression of Human ACE2 in HEK293 enables efficient detection and study of its functions.

United Kingdom

2020-02-22

Viral Host Research

The Human ACE2 Stable Cell Line - HEK293 provides an experimental platform for studying the interaction between coronaviruses and host cells.

French

2020-08-07

Drug Development Aid

This cell line assists in screening therapeutic drugs targeting ACE2-mediated viral infections.

United Kingdom

2020-11-01

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Human ACE2 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Background

Case Study

Applications

Q & A

Customer Reviews

What is the background of the establishment of the Human ACE2 Stable Cell Line - HEK293? How does this cell line meet the needs of current research fields?

In which specific research areas does the Human ACE2 Stable Cell Line - HEK293 demonstrate its unique value?

What is the significance of the establishment of the Human ACE2 Stable Cell Line - HEK293 for future vaccine development?

What are the advantages of the Human ACE2 Stable Cell Line - HEK293 in simulating the natural infection process?

What are the key technical challenges in the establishment of the Human ACE2 Stable Cell Line - HEK293, and how were these challenges overcome?

Efficient research

Efficient detection

Viral Host Research

Drug Development Aid

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