Cat.No. : CSC-SC015861-M1 Host Cell: CHO-K1
| Cat. No. | CSC-SC015861-M1 |
| Description | This cell line is engineered to stably overexpress mouse Tgfb1 (transforming growth factor, beta 1). |
| Gene | Tgfb1 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Transforming growth factor beta 1 (TGFB1) is a potent cytokine that plays a crucial role in driving processes such as development, fibrosis, and cancer. Researchers have unveiled a novel intracellular secretion pathway for TGFB1, a potent cytokine crucial in development, fibrosis, and cancer. This pathway involves fibroblast-ECM communication mediated by ILK, which regulates the release of latent TGFB1 from fibroblasts. The secretion process requires interaction with ARHGAP26/GRAF1 to restrict RHOA activity and involves GORASP2/GRASP55 and autophagosomal intermediates. TGFB1 secretion relies on the autophagic machinery, highlighting a unique mode of secretion that controls its bioavailability and activity, crucial for cellular processes implicated in fibrosis and cancer.
Figure 1. In Ilk cKO murine fibroblasts, intracellular accumulation of TGFB1 large latent complex was observed by researchers. Disruption of FBN1 and LTBP1 fiber formation in the extracellular space occurred due to Ilk deletion, while there was an increase in intracellular LTBP1-TGFB1 complex levels. NPR3 function remained unaffected. (Nüchel J, et al., 2018)
Utilizing Creative Biogene's Mouse Tgfb1 Stable Cell Line - CHO-K1 could enhance similar experiments by providing a consistent and reliable cellular model for studying the effects of TGFB1 in a controlled environment. This stable cell line allows for the investigation of TGFB1-mediated signaling pathways, cellular responses, and potential therapeutic interventions with greater precision and reproducibility.
A: CHO-K1 cells were likely chosen for their ability to express recombinant proteins efficiently, making them suitable for studying Tgfb1 secretion and its role in cellular signaling and biological processes.
A: Stability was likely confirmed through methods such as ELISA, immunoblotting, or functional assays assessing Tgfb1-mediated signaling pathways, with continuous selection pressure applied.
A: Characterization may involve analysis of Tgfb1 secretion levels, bioactivity assays, downstream signaling cascades, and functional implications in cell proliferation, differentiation, and immune regulation.
A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with in vivo models or clinical data helps validate the relevance of Tgfb1 expression in various biological processes and diseases such as fibrosis, cancer, and autoimmune disorders.
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This Mouse Tgfb1 Stable Cell Line is a game-changer! Its stable expression in CHO-K1 cells has simplified my research on TGF-β signaling pathways.
Using this cell line feels like having a magic wand! The stable Tgfb1 expression has made studying cell growth and differentiation mechanisms a breeze.
Can't believe how much smoother my experiments have become with this cell line! Its reliable expression in CHO-K1 cells has brought clarity to my research on fibrosis.
So impressed with this Mouse Tgfb1 Stable Cell Line! Its consistent expression has given me confidence in my findings and propelled my research forward.
Huge shoutout to this cell line for simplifying my work! With stable Tgfb1 expression, I can delve deeper into TGF-β signaling without any worries.
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