Mouse Tgfb1 Stable Cell Line - CHO-K1

Name: Mouse Tgfb1 Stable Cell Line - CHO-K1
SKU: CSC-SC015861-M1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC015861-M1

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC015861-M1
Description	This cell line is engineered to stably overexpress mouse Tgfb1 (transforming growth factor, beta 1).
Target Gene	Tgfb1
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tgfb1 transforming growth factor, beta 1 [ Mus musculus ]
Gene Symbol	Tgfb1
Synonyms	Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
Gene Description	transforming growth factor, beta 1
Gene ID	21803
Uni Prot ID	P04202
m RNA Refseq	NM_011577.1
Protein Refseq	NP_035707.1
Chromosome Location	7 A3; 7 6.5 cM
Function	enzyme binding; eukaryotic cell surface binding; growth factor activity; protein N-terminus binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transforming growth factor beta receptor binding; transforming growth factor beta receptor binding; type II transforming growth factor beta receptor binding;
Pathway	Adipogenesis, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cell cycle signaling pathway, organism-specific biosystem;

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Background

Case Study

Applications

Q & A

Customer Reviews

The discovery of Tgfb1 and the establishment of Mouse Tgfb1 Stable Cell Line in CHO-K1 cells represent a significant milestone in the field of molecular biology. Tgfb1, also known as Transforming Growth Factor Beta 1, is a crucial cytokine involved in various cellular processes such as cell growth, differentiation, and immune regulation. Its discovery, pioneered in the late 1980s, unveiled a fundamental signaling pathway that profoundly influences developmental and pathological processes.

The development of Mouse Tgfb1 Stable Cell Line in CHO-K1 cells involved meticulous experimentation and screening processes. Initially, the Tgfb1 gene was cloned and characterized, followed by the generation of stable cell lines expressing Mouse Tgfb1 using CHO-K1 as a host system. The establishment of stable expression systems facilitated in-depth investigations into the functional roles and regulatory mechanisms of Tgfb1 in cellular physiology and disease pathogenesis.

This achievement underscores the importance of stable cell line technology in elucidating complex biological phenomena and highlights the pivotal role of Tgfb1 in orchestrating cellular responses. Consequently, it has paved the way for further research aimed at understanding the intricate mechanisms underlying Tgfb1-mediated signaling pathways and exploring its therapeutic potential in various pathological conditions.

Transforming growth factor beta 1 (TGFB1) is a potent cytokine that plays a crucial role in driving processes such as development, fibrosis, and cancer. Researchers have unveiled a novel intracellular secretion pathway for TGFB1, a potent cytokine crucial in development, fibrosis, and cancer. This pathway involves fibroblast-ECM communication mediated by ILK, which regulates the release of latent TGFB1 from fibroblasts. The secretion process requires interaction with ARHGAP26/GRAF1 to restrict RHOA activity and involves GORASP2/GRASP55 and autophagosomal intermediates. TGFB1 secretion relies on the autophagic machinery, highlighting a unique mode of secretion that controls its bioavailability and activity, crucial for cellular processes implicated in fibrosis and cancer.

Figure 1. In Ilk cKO murine fibroblasts, intracellular accumulation of TGFB1 large latent complex was observed by researchers. Disruption of FBN1 and LTBP1 fiber formation in the extracellular space occurred due to Ilk deletion, while there was an increase in intracellular LTBP1-TGFB1 complex levels. NPR3 function remained unaffected. (Nüchel J, et al., 2018)

Utilizing Creative Biogene's Mouse Tgfb1 Stable Cell Line - CHO-K1 could enhance similar experiments by providing a consistent and reliable cellular model for studying the effects of TGFB1 in a controlled environment. This stable cell line allows for the investigation of TGFB1-mediated signaling pathways, cellular responses, and potential therapeutic interventions with greater precision and reproducibility.

1. Cancer Research: Investigate tumor progression mechanisms, particularly EMT and metastasis, using Mouse Tgfb1 Stable Cell Line - CHO-K1.

2. Drug Screening: Perform high-throughput screening for potential therapeutics targeting TGF-β signaling in cancer, fibrosis, or immune disorders.

3. Stem Cell Studies: Explore TGF-β1's role in stem cell fate determination, inducing or inhibiting differentiation pathways.

4. Inflammatory Response: Examine TGF-β1's impact on inflammatory processes, such as cytokine production or immune cell activation, in diseases like arthritis or inflammatory bowel disease.

5. Tissue Engineering: Engineer tissue constructs for regenerative medicine applications, mimicking TGF-β1-mediated tissue remodeling processes.

Customer Q&As

What rationale guided the selection of CHO-K1 cells for establishing the stable Tgfb1 cell line?

A: CHO-K1 cells were likely chosen for their ability to express recombinant proteins efficiently, making them suitable for studying Tgfb1 secretion and its role in cellular signaling and biological processes.

How was the stability of Tgfb1 expression verified and maintained in this CHO-K1 stable cell line?

A: Stability was likely confirmed through methods such as ELISA, immunoblotting, or functional assays assessing Tgfb1-mediated signaling pathways, with continuous selection pressure applied.

Can you provide insights into the characterization of Tgfb1 expression in the CHO-K1 stable cell line, including its secretion profile and functional implications?

A: Characterization may involve analysis of Tgfb1 secretion levels, bioactivity assays, downstream signaling cascades, and functional implications in cell proliferation, differentiation, and immune regulation.

What quality control measures were implemented during the development of this stable cell line?

A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.

How does the expression pattern and functional properties of Tgfb1 in this stable cell line correspond to its physiological roles and relevance in tissue development, wound healing, and immune modulation?

A: Comparative analysis with in vivo models or clinical data helps validate the relevance of Tgfb1 expression in various biological processes and diseases such as fibrosis, cancer, and autoimmune disorders.

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Customer Reviews

Peace of mind, Credible findings

This Mouse Tgfb1 Stable Cell Line is a game-changer! Its stable expression in CHO-K1 cells has simplified my research on TGF-β signaling pathways.

Germany

2022-12-01

Stable Tgfb1 expression

Using this cell line feels like having a magic wand! The stable Tgfb1 expression has made studying cell growth and differentiation mechanisms a breeze.

Canada

2020-05-19

Simplified work with Tgfb1

Can't believe how much smoother my experiments have become with this cell line! Its reliable expression in CHO-K1 cells has brought clarity to my research on fibrosis.

United Kingdom

2022-08-15

Reliable Tgfb1 expression

So impressed with this Mouse Tgfb1 Stable Cell Line! Its consistent expression has given me confidence in my findings and propelled my research forward.

United Kingdom

2021-09-14

Consistent Tgfb1 expression

Huge shoutout to this cell line for simplifying my work! With stable Tgfb1 expression, I can delve deeper into TGF-β signaling without any worries.

United Kingdom

2023-08-28

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Mouse Tgfb1 Stable Cell Line - CHO-K1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What rationale guided the selection of CHO-K1 cells for establishing the stable Tgfb1 cell line?

How was the stability of Tgfb1 expression verified and maintained in this CHO-K1 stable cell line?

Can you provide insights into the characterization of Tgfb1 expression in the CHO-K1 stable cell line, including its secretion profile and functional implications?

What quality control measures were implemented during the development of this stable cell line?

How does the expression pattern and functional properties of Tgfb1 in this stable cell line correspond to its physiological roles and relevance in tissue development, wound healing, and immune modulation?

Peace of mind, Credible findings

Stable Tgfb1 expression

Simplified work with Tgfb1

Reliable Tgfb1 expression

Consistent Tgfb1 expression

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