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Cat.No. : CSC-RO0901 Host Cell: P3U1
| Cat. No. | CSC-RO0901 |
| Description | This cell line is engineered to stably overexpress human TGFB1. |
| Gene | TGFB1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | P3U1 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human TGFB1 Stable Cell Line - P3U1 was established by introducing the human TGFB1 gene (Transforming Growth Factor Beta 1) into the P3U1 cell line. P3U1 is a mouse B-cell line used for immunological and cell biology research. The establishment of this cell line involves genetic engineering techniques to introduce and express the human TGFB1 gene within the P3U1 cells.
A: The stability of TGFB1 expression in the Human TGFB1 Stable Cell Line - P3U1 is typically maintained through stable gene expression experiments. This stability can be ensured by selective pressure (such as drug selection). Once the TGFB1 gene is successfully introduced and stably expressed in the P3U1 cells, appropriate selective pressures will continue to maintain its expression to ensure the stability of the cell line.
A: TGFB1 is a cytokine that plays a crucial role in the immune system. It regulates immune responses, cell proliferation, cell differentiation, and immune tolerance. Establishing a TGFB1 stable cell line is essential for in-depth research into the role of TGFB1 in these immunological processes because it provides a controlled experimental platform for studying the molecular mechanisms and effects of TGFB1.
A: TGFB1 has a complex role in cancer research. It can have dual properties, both inhibiting tumor growth and promoting tumor invasion and metastasis. TGFB1 can inhibit cell proliferation and induce apoptosis, thereby exerting anti-tumor effects. However, in late-stage cancer, it may also facilitate tumor immune evasion and suppress immune responses. Using the TGFB1 Stable Cell Line - P3U1, researchers can study the role of TGFB1 at different stages of cancer development and explore potential therapeutic strategies.
A: Yes, the Human TGFB1 Stable Cell Line - P3U1 can be used to model TGFB1-mediated immune suppression. This cell line stably expresses TGFB1, allowing researchers to study how TGFB1 affects immune cell activation, proliferation, apoptosis, and immune responses. Through experiments in this cell line, researchers can simulate and investigate the role of TGFB1 in immune suppression, gaining insights into its mechanisms and potential therapeutic strategies.
A: The specific mechanisms of TGFB1 in immune regulation involve multiple levels. It can suppress the activation and proliferation of T cells and other immune cells, reducing the functionality of immune cells. Additionally, TGFB1 can induce the differentiation of immune cells into immunosuppressive types, such as regulatory T cells (Treg cells). Through these mechanisms, TGFB1 contributes to maintaining immune tolerance and preventing excessive immune responses.
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