Mouse Tgfb1 Stable Cell Line - Nme

Name: Mouse Tgfb1 Stable Cell Line - Nme
SKU: CSC-SC015861-M2
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC015861-M2

Host Cell : Nme Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC015861-M2
Description	This cell line is engineered to stably overexpress mouse transforming growth factor, beta 1 (Tgfb1, also known as Tgfb; Tgfb-1; TGFbeta; TGF-beta; TGFbeta1; TGF-beta1).
Background	The stable cell line Tgfb1 in mice Transforming Growth Factor Beta 1 (TGF-β1), a secreted ligand that is a member of the TGF-beta class of proteins, is expressed in the Nme cell line. Numerous biological processes, such as immune system function, cell proliferation, and differentiation, are influenced by the mouse Tgfb1 gene. It is found on chromosome 7 and encodes a preproprotein that is digested by proteases to produce a mature peptide and a latency-associated peptide (LAP). By interacting to different TGF-beta receptors, TGF-β1 controls the expression of genes by attracting and activating transcription factors from the SMAD family.
Target Gene	Tgfb1
Gene Species	Mus musculus (Mouse)
Host Cell	Nme
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tgfb1 transforming growth factor, beta 1 [ Mus musculus ]
Gene Symbol	Tgfb1
Synonyms	Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
Gene Description	transforming growth factor, beta 1
Gene ID	21803
Uni Prot ID	P04202
m RNA Refseq	NM_011577.1
Protein Refseq	NP_035707.1
Chromosome Location	7 A3; 7 6.5 cM
Function	enzyme binding; eukaryotic cell surface binding; growth factor activity; protein N-terminus binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transforming growth factor beta receptor binding; transforming growth factor beta receptor binding; type II transforming growth factor beta receptor binding;
Pathway	Adipogenesis, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cell cycle signaling pathway, organism-specific biosystem;

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Applications

Q & A

Customer Reviews

1. Immunological response research: Nme cells are used to study immune system function and how TGF-β1 influences immune modulation.

2. Inflammation studies: These cells help in understanding inflammation mechanisms and the role of TGF-β1 in inflammatory responses.

3. Bone remodeling research: Nme cells are essential for examining TGF-β1's effect on bone remodeling and skeletal development.

4. Cell proliferation and differentiation: The stable Tgfb1 cell line aids in exploring how TGF-β1 controls cell growth and differentiation processes.

5. Therapeutic development: Insights into TGF-β1 signaling pathways in Nme cells can guide the development of treatments for diseases with dysregulated TGF-β1 activity.

Customer Q&As

What is the expression level of this cell line during the expression of Tgfb1?

A: Mouse Tgfb1 Stable Cell Line-Nme cell line shows high level and stable gene expression when expressing Tgfb1 gene. This was verified by qPCR and Western Blotting analysis, showing that Tgfb1 mRNA and protein remained stably expressed over multiple passages. In addition, functional analysis, such as TGFβ1-induced activation of downstream signaling pathways, further proved the functional expression level of this gene. This cell line is suitable for studying the role of TGFβ1 in cell growth, differentiation, apoptosis and immune regulation. Specific experiments include: evaluating the role of TGFβ1 in fibrotic diseases, studying its function in the tumor microenvironment, and exploring the mechanism of TGFβ1 in immune suppression and immune evasion. In addition, this cell line is also suitable for screening and validating drugs targeting the TGFβ1 signaling pathway.

What are the specific methods for experimental validation of this cell line?

A: Experimental validation methods for this cell line include gene expression analysis (qPCR) to confirm Tgfb1 mRNA levels, protein analysis (Western Blotting and ELISA) to detect TGFβ1 protein levels, and functional experiments (such as cell proliferation and migration assays) to evaluate TGFβ1 biological effects. In addition, reporter gene analysis can also be used to study the activation of downstream signaling pathways induced by TGFβ1, such as the phosphorylation status of Smad protein.

Is this cell line suitable for functional studies in vitro and in vivo?

A: Mouse Tgfb1 Stable Cell Line-Nme is not only suitable for in vitro functional studies, such as cell proliferation, migration and signaling pathway activation experiments, but can also be used in in vivo research models. This cell line showed expected TGF-β1 expression and related biological effects, such as tumor growth regulation and immune evasion, in transplantation experiments in mice. These characteristics make it an important tool for studying the biological functions and therapeutic targets of TGF-β1.

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Customer Reviews

Good stability

This supplier's Tgfb1 stable cell line appears to be very stable. During the culture process, I noticed that the cells grew very consistently and their gene expression was very stable, making them ideal for studying immune responses and inflammation. The supplier's service is also very timely and I am very satisfied.

Germany

2022-07-06

High service quality

I purchased the Tgfb1 stable cell line from this platform. The cells grew rapidly and stably, and the experimental results were very consistent. The customer service staff had a very good attitude and solved many of my problems. The shopping experience was very pleasant.

United Kingdom

2023-09-16

Fast shipping

The performance of this Tgfb1 stable cell line exceeded my expectations. The cells adhered well to the culture dish, grew rapidly, and had stable gene expression. The delivery on the platform is also very fast. I will come again next time to buy other products.

United States

2021-01-28

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Mouse Tgfb1 Stable Cell Line - Nme

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Applications

Q & A

Customer Reviews

What is the expression level of this cell line during the expression of Tgfb1?

What are the specific methods for experimental validation of this cell line?

Is this cell line suitable for functional studies in vitro and in vivo?

Good stability

High service quality

Fast shipping

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