Cat.No. : CSC-SC015861-M2 Host Cell: Nme
| Cat. No. | CSC-SC015861-M2 |
| Description | This cell line is engineered to stably overexpress mouse transforming growth factor, beta 1 (Tgfb1, also known as Tgfb; Tgfb-1; TGFbeta; TGF-beta; TGFbeta1; TGF-beta1). |
| Background | The stable cell line Tgfb1 in mice Transforming Growth Factor Beta 1 (TGF-β1), a secreted ligand that is a member of the TGF-beta class of proteins, is expressed in the Nme cell line. Numerous biological processes, such as immune system function, cell proliferation, and differentiation, are influenced by the mouse Tgfb1 gene. It is found on chromosome 7 and encodes a preproprotein that is digested by proteases to produce a mature peptide and a latency-associated peptide (LAP). By interacting to different TGF-beta receptors, TGF-β1 controls the expression of genes by attracting and activating transcription factors from the SMAD family. |
| Gene | Tgfb1 |
| Host Cell | Nme |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Mouse Tgfb1 Stable Cell Line-Nme cell line shows high level and stable gene expression when expressing Tgfb1 gene. This was verified by qPCR and Western Blotting analysis, showing that Tgfb1 mRNA and protein remained stably expressed over multiple passages. In addition, functional analysis, such as TGFβ1-induced activation of downstream signaling pathways, further proved the functional expression level of this gene. This cell line is suitable for studying the role of TGFβ1 in cell growth, differentiation, apoptosis and immune regulation. Specific experiments include: evaluating the role of TGFβ1 in fibrotic diseases, studying its function in the tumor microenvironment, and exploring the mechanism of TGFβ1 in immune suppression and immune evasion. In addition, this cell line is also suitable for screening and validating drugs targeting the TGFβ1 signaling pathway.
A: Experimental validation methods for this cell line include gene expression analysis (qPCR) to confirm Tgfb1 mRNA levels, protein analysis (Western Blotting and ELISA) to detect TGFβ1 protein levels, and functional experiments (such as cell proliferation and migration assays) to evaluate TGFβ1 biological effects. In addition, reporter gene analysis can also be used to study the activation of downstream signaling pathways induced by TGFβ1, such as the phosphorylation status of Smad protein.
A: Mouse Tgfb1 Stable Cell Line-Nme is not only suitable for in vitro functional studies, such as cell proliferation, migration and signaling pathway activation experiments, but can also be used in in vivo research models. This cell line showed expected TGF-β1 expression and related biological effects, such as tumor growth regulation and immune evasion, in transplantation experiments in mice. These characteristics make it an important tool for studying the biological functions and therapeutic targets of TGF-β1.
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This supplier's Tgfb1 stable cell line appears to be very stable. During the culture process, I noticed that the cells grew very consistently and their gene expression was very stable, making them ideal for studying immune responses and inflammation. The supplier's service is also very timely and I am very satisfied.
I purchased the Tgfb1 stable cell line from this platform. The cells grew rapidly and stably, and the experimental results were very consistent. The customer service staff had a very good attitude and solved many of my problems. The shopping experience was very pleasant.
The performance of this Tgfb1 stable cell line exceeded my expectations. The cells adhered well to the culture dish, grew rapidly, and had stable gene expression. The delivery on the platform is also very fast. I will come again next time to buy other products.
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