Human LILRB4 Stable Cell Line - HEK293T

Name: Human LILRB4 Stable Cell Line - HEK293T
SKU: CSC-RO0666
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0666

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0666
Description	This cell line is engineered to overexpress human leukocyte immunoglobulin like receptor B4 (LILRB4).
Target Gene	LILRB4
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LILRB4 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4 [ Homo sapiens ]
Gene Symbol	LILRB4
Synonyms	HM18; ILT3; LIR5; CD85K; LIR-5; LILRB5
Gene Description	leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
Gene ID	11006
Uni Prot ID	Q8NHJ6
m RNA Refseq	NM_006847.3
Protein Refseq	NP_006838.3
Chromosome Location	19q13.4
Function	antigen binding; protein binding; receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem;
MIM	604821

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Background

Case Study

Applications

Q & A

Customer Reviews

The Human LILRB4 (Leukocyte Immunoglobulin-Like Receptor B4) Stable Cell Line is engineered to express the LILRB4 gene, which encodes a protein involved in immune cell interactions and is implicated in the regulation of immune responses. LILRB4 has been associated with various immune-related diseases, including autoimmune disorders and cancer.

The HEK293T cell line, a derivative of the HEK293 cell line, is selected for its high transfection efficiency and its ability to support the expression of complex proteins. When engineered to stably express the human LILRB4 gene, this cell line serves as a valuable tool for studying the function of LILRB4 in immune cell signaling and its potential role in disease. This cell line is instrumental for drug screening and the development of therapeutic strategies targeting LILRB4.

Acute monocytic leukemia (AML) is a highly aggressive subtype of leukemia, characterized by the expression of immune checkpoint proteins like leukocyte immunoglobulin-like receptor B4 (LILRB4). This receptor plays a crucial role in immune evasion, making it a significant target for novel immunotherapies. In recent research, a humanized monoclonal IgG1 LILRB4-blocking antibody (h128-3) demonstrated promising results by improving immune regulation while reducing LILRB4 expression by 40–60%. However, its effectiveness was influenced by the interaction between the antibody's Fc region and Fc gamma receptors (FcγRs), highlighting the importance of this pathway in therapeutic design. The study further explored the mechanism of FcγR-dependent antigenic modulation, elucidating how inhibiting or removing FcγRI impacted the blocking function of h128-3, thus affecting T-cell-mediated cytotoxicity.

Figure 1 illustrates that h128-3-induced internalization of LILRB4 in FcγR high monocytic AML cells is enhanced through crosslinking of FcγRI. (doi: 10.1093/abt/tbad025) Figure 1. The researchers employed flow cytometry to analyze LILRB4 and FcγRI expression in HEK293T LILRB4/FcγRI overexpressing cells, assessing h128-3-induced LILRB4 internalization in monocytic AML. (Morse JW, et al., 2023)

Creative Biogene's Human LILRB4 Stable Cell Line - HEK293T offers a reliable platform for researchers investigating immune checkpoint mechanisms in myeloid malignancies. This cell line can be genetically modified using lentivirus to express human LILRB4, either alone or in conjunction with FcγRI. The cells are rigorously selected to ensure stable expression, providing a valuable tool for studying the functional dynamics of LILRB4 and FcγR interactions in vitro.

The Human LILRB4 Stable Cell Line - HEK293T is a cell line that expresses the LILRB4 gene, which is involved in immune cell activation and signaling. This cell line is useful for studying the role of LILRB4 in immune responses and for the development of therapeutics targeting this protein.

（1）Immunology Research and LILRB4 Function:

LILRB4 is a member of the leukocyte immunoglobulin-like receptor (LILR) family and plays a role in immune cell function. This cell line can be used to investigate the biological functions of LILRB4 and its role in modulating immune responses.

（2）Drug Development for Immune-Related Diseases:

Given the role of LILRB4 in immune cell activation, this cell line can be used to screen for compounds that modulate LILRB4 activity, potentially leading to the development of new therapeutics for immune-related diseases.

（3）Study of LILRB4 Signaling Pathways:

The cell line can be utilized to study the intracellular signaling pathways activated by LILRB4 and to identify potential targets for modulating immune responses, which could be relevant for the treatment of autoimmune diseases and cancer immunotherapy.

Customer Q&As

What advantages does the Human LILRB4 Stable Cell Line - HEK293T offer for the study of immune checkpoint regulation?

A: The Human LILRB4 Stable Cell Line - HEK293T provides a robust model for investigating the role of LILRB4, an immune inhibitory receptor, in immune checkpoint regulation. This cell line allows for the detailed analysis of LILRB4 signaling pathways, ligand interactions, and its impact on immune cell function, particularly in the context of cancer immunotherapy. The use of a stable HEK293T cell line expressing LILRB4 facilitates consistent and reproducible results, essential for advancing our understanding of immune checkpoint mechanisms and developing novel therapeutic interventions.

How can the Human LILRB4 Stable Cell Line - HEK293T be utilized to screen for antibodies or small molecules that modulate LILRB4 activity?

A: Researchers can employ the Human LILRB4 Stable Cell Line - HEK293T in high-throughput screening assays to identify antibodies or small molecules that bind to LILRB4, potentially modulating its activity. These screenings involve measuring changes in downstream signaling events, such as calcium flux, phosphorylation states of signaling molecules, or reporter gene activation, in response to compound treatment. Such studies are critical for discovering new drugs that can either enhance or inhibit LILRB4 signaling, offering therapeutic options for diseases where LILRB4 plays a key regulatory role.

What measures should be implemented when culturing the Human LILRB4 Stable Cell Line - HEK293T to maintain its stability and expression of LILRB4?

A: To maintain the stability and expression of LILRB4 in the Human LILRB4 Stable Cell Line - HEK293T, it's crucial to culture the cells under optimal conditions, including the correct temperature (37°C), CO2 levels (5%), and using a medium supplemented with the appropriate selective antibiotics. Periodic verification of LILRB4 expression through flow cytometry or Western blot analysis is recommended to ensure consistent expression. Additionally, avoiding overgrowth and subculturing cells before reaching confluency will help maintain the cell line's integrity and expression levels.

In the context of immune-oncology research, how can the Human LILRB4 Stable Cell Line - HEK293T advance our understanding of tumor-immune evasion?

A: The Human LILRB4 Stable Cell Line - HEK293T can significantly contribute to immune-oncology research by facilitating studies on how tumor cells exploit LILRB4 to evade immune detection and destruction. By expressing LILRB4 on HEK293T cells, researchers can mimic tumor cell interactions with immune cells, studying how LILRB4 engagement suppresses immune responses. Investigating the blockade or enhancement of LILRB4 signaling in this cell line could uncover new strategies for cancer immunotherapy, aiming to boost the immune system's ability to recognize and eliminate tumor cells.

What experimental approaches can be taken to study the ligand-receptor interactions involving LILRB4 using the Human LILRB4 Stable Cell Line - HEK293T?

A: To study ligand-receptor interactions involving LILRB4, researchers can employ several experimental approaches using the Human LILRB4 Stable Cell Line - HEK293T, including co-immunoprecipitation (Co-IP) to identify physical interactions between LILRB4 and its ligands. Surface plasmon resonance (SPR) and bio-layer interferometry (BLI) are additional biophysical methods that can quantify the binding affinity and kinetics of these interactions. Furthermore, functional assays, such as reporter gene assays or cytokine release assays, can assess the biological consequences of ligand binding to LILRB4, providing insights into how these interactions regulate immune responses.

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Customer Reviews

Accurate LILRB4 Expression

The Human LILRB4 Stable Cell Line - HEK293T ensures accurate and consistent expression of the LILRB4 receptor. This accuracy is critical for reliable investigation of LILRB4's role in immune modulation and receptor signaling, providing a solid basis for experimental reproducibility.

United States

2021-06-01

High Transfection Efficiency

HEK293T cells, known for their high transfection efficiency, make the Human LILRB4 Stable Cell Line - HEK293T exceptionally receptive to further genetic modifications. This trait is beneficial for us looking to introduce additional genes or reporters into the cell line.

French

2023-06-02

Robust Cell Growth

The Human LILRB4 Stable Cell Line - HEK293T benefits from the robust growth characteristics of HEK293T cells, facilitating easier scaling of experiments. This robust growth is advantageous for high-volume experimental setups, such as drug screening or large-scale studies.

Germany

2020-03-30

Enhanced Data Reliability

Stable expression of LILRB4 in the Human LILRB4 Stable Cell Line - HEK293T enhances the reliability of data, especially in studies focusing on the immune checkpoint functions of LILRB4. Reliable data is crucial for forming valid conclusions and advancing scientific knowledge.

Canada

2021-09-07

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Human LILRB4 Stable Cell Line - HEK293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What advantages does the Human LILRB4 Stable Cell Line - HEK293T offer for the study of immune checkpoint regulation?

How can the Human LILRB4 Stable Cell Line - HEK293T be utilized to screen for antibodies or small molecules that modulate LILRB4 activity?

What measures should be implemented when culturing the Human LILRB4 Stable Cell Line - HEK293T to maintain its stability and expression of LILRB4?

In the context of immune-oncology research, how can the Human LILRB4 Stable Cell Line - HEK293T advance our understanding of tumor-immune evasion?

What experimental approaches can be taken to study the ligand-receptor interactions involving LILRB4 using the Human LILRB4 Stable Cell Line - HEK293T?

Accurate LILRB4 Expression

High Transfection Efficiency

Robust Cell Growth

Enhanced Data Reliability

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