Cat.No. : CSC-RO0547 Host Cell: HEK293T
| Cat. No. | CSC-RO0547 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human FOLH1. |
| Gene | FOLH1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Four new murine monoclonal antibodies (mAbs) that target FOLH1, a verified marker in prostate cancer, were described by the researchers. A variety of in vitro tests, including as Western blotting, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, and surface plasmon resonance, were used to assess the mAbs. They evaluated the effectiveness of mAbs for in vivo applications using a mouse xenograft model. The findings show that all mAbs for FOLH1 have a high level of specificity, with 1A11 and 3F11 binding linear epitopes and 5D3 and 5B1 identifying unique conformational epitopes. Interestingly, 5D3 outperformed J591 in terms of affinity (0.14 nM by ELISA, 1.2 nM by flow cytometry), indicating that it may be used in more advanced theranostic development that targets FOLH1.
Figure 1. The researchers achieved stable overexpression of human FOLH1 (also known as PSMA) in HEK293T/17 cells via transfection with pcDNA4/V5-His A vector carrying FOLH1 cDNA. Peptide scanning was used by the researchers to demonstrate FOLH1 epitope identification across species. Using ortholog/paralog samples and human PSMA-overexpressing HEK293T/17 lysates, they further confirmed with mAb Western blotting. (Nováková Z, et al., 2017)
A: The Human FOLH1 Stable Cell Line - HEK293T, by stably expressing human folylpolyglutamate synthase (FOLH1), offers an ideal model for studying the folate metabolic pathway. This cell line allows scientists to directly observe FOLH1 activity at the cellular level and its impact on folate metabolism. By manipulating FOLH1 expression levels, researchers can explore the link between folate metabolism abnormalities and diseases such as cancer, as well as develop therapeutic strategies targeting the folate metabolic pathway.
A: In drug development, the Human FOLH1 Stable Cell Line - HEK293T can serve as a screening platform to identify and validate compounds that inhibit the growth of folate-dependent tumors. By testing compounds in these cells, researchers can evaluate their effects on FOLH1 activity, thereby screening for potential anticancer drug candidates. This approach helps accelerate the development of novel anticancer therapies targeting the folate metabolic pathway.
A: The Human FOLH1 Stable Cell Line - HEK293T contributes to the study of the molecular mechanisms of folate deficiency by simulating a cellular environment where folate is lacking. This cell line can be used to investigate how folate deficiency affects cellular functions and metabolic disorders, as well as how these disorders impact cell growth, differentiation, and apoptosis. This is crucial for understanding the pathophysiology of folate deficiency and developing preventive strategies.
A: The Human FOLH1 Stable Cell Line - HEK293T can be used to evaluate the bioavailability of different folate supplements by monitoring changes in intracellular folate levels. This cell line assists researchers in understanding the conversion and utilization efficiency of various folate forms within cells, providing a scientific basis for the development of more effective folate supplements.
A: Folate plays a crucial role in neural development, especially during embryonic stages. The Human FOLH1 Stable Cell Line - HEK293T can simulate the environment of neural cells to study how folate affects the growth and development of neural cells. This is significant for uncovering the link between folate deficiency and neural developmental abnormalities, as well as developing strategies to prevent neural developmental disorders.
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The Human FOLH1 Stable Cell Line - HEK293T can efficiently and stably express the FOLH1 gene, facilitating its functional study.
This cell line grows rapidly and is easy to cultivate and maintain under laboratory conditions.
Suitable for various biological research, including drug screening and gene function analysis.
HEK293T cells are sensitive to multiple transfection methods, facilitating gene manipulation.
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