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scFv(PSMA)-CD28-41BB-CD3zeta CAR-T Lentivirus

For research use only. Not intended for any clinical use.

Cat. No. :   LVG00065Z

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

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Gene Information

Cat. No. LVG00065Z
Description Lentivirus particles containing third generation of anti-PSMA CAR (chimeric antigen receptor) scFv-CD28-41BB-CD3zeta.
Gene FOLH1
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name FOLH1 folate hydrolase (prostate-specific membrane antigen) 1 [ Homo sapiens ]
Gene Symbol FOLH1
Synonyms PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
Gene Description folate hydrolase (prostate-specific membrane antigen) 1
Gene ID 2346
Uni Prot ID Q04609
m RNA Refseq NM_001014986.1
Protein Refseq NP_001014986.1
Chromosome Location 11p11.2
Function carboxypeptidase activity; dipeptidase activity; metal ion binding; metallopeptidase activity; peptidase activity;
Pathway One Carbon Metabolism, organism-specific biosystem; Vitamin digestion and absorption, organism-specific biosystem; Vitamin digestion and absorption, conserved biosystem;
MIM 600934
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Highly recommend

This CAR-T lentivirus construct showed remarkable targeting efficiency against PSMA+ tumors in our preclinical models. The dual co-stimulatory domains (CD28/4-1BB) enhanced persistence and cytotoxicity. Highly recommended!

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