Panoply™ Human TNFRSF18 Over-expressing Stable Cell Line

Name: Panoply™ Human TNFRSF18 Over-expressing Stable Cell Line
SKU: CSC-SC016361
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016361

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC016361
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TNFRSF18
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF18 tumor necrosis factor receptor superfamily, member 18 [ Homo sapiens ]
Gene Symbol	TNFRSF18
Synonyms	AITR; GITR; CD357; GITR-D
Gene Description	tumor necrosis factor receptor superfamily, member 18
GeneID	8784
Uni ProtID	Q9Y5U5
mRNA Refseq	NM_148901.1
Protein Refseq	NP_683699.1
Chromosome Location	1p36.3
Function	tumor necrosis factor-activated receptor activity;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
MIM	603905

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Colorectal cancer (CRC) is one of the most common digestive system malignancies worldwide and is associated with poor survival outcomes. CD8⁺ T cell exhaustion plays a crucial role in promoting tumor immune evasion. However, the dynamic evolution of CD8⁺ T cell exhaustion in CRC patients with different TNM (tumor-node-metastasis) stages and its impact on clinical prognosis remain incompletely elucidated. Here, researchers revealed how TNM stage in CRC patients influences CD8⁺ T cell exhaustion through differential TNFRSF18/CXCL13 dynamics and ribosomal stemness. TNFRSF18 expression is significantly higher in T cells infiltrating tumor tissue than in adjacent non-tumor areas, and CD8⁺ T cells with high TNFRSF18 expression exhibit distinct exhaustion characteristics. During colorectal cancer progression, TNM-stage-driven remodelling of the tumour microenvironment (TME) induced progressive CD8⁺ T cell exhaustion marked by declining TNFRSF18 and rising CXCL13 expression in tumour-infiltrating T cells elevation of both markers in the tumour compared with adjacent tissues. Furthermore, the researchers found that tumor cells exhibited elevated expression of stem cell-associated ribosomal genes (RPS7, RPL8, RPL30), peaking at T4 stage and associated with poor prognosis and immune evasion.

In CD4⁺ T cells, TNFRSF18-positive expression promoted TNF binding to TNFR2 and activated the atypical NF-κB pathway (Figure 1D), while CXCL13-positive expression enhances PD-1 signaling. Both TNFRSF18 and CXCL13 are key regulators of T cell function and influence the TNF signaling pathway. The researchers then generated a TNFRSF18-overexpressing Jurkat T cell line and performed RT-qPCR to assess the effect of TNFRSF18 overexpression on T cell activation in Jurkat cells. As shown in Figures 1E and 1F, TNFRSF18-overexpressing Jurkat cells significantly reduced TNF-α and IFN-γ mRNA levels, indicating that TNFRSF18 inhibits T cell effector cytokine production, supporting its role in promoting T cell exhaustion.

Figure 1. The immune regulatory role of TNFRSF18 in T cells was investigated through overexpression experiments. (Jia T, et al., 2025)

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