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Cat.No. : CSC-RO0563 Host Cell: HEK293T
| Cat. No. | CSC-RO0563 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Dog TNFRSF18. |
| Gene | TNFRSF18 |
| Gene Species | Canis lupus familiaris (Dog) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Try changing the culture medium or coating culture plates with cell adhesion promoters such as collagen or fibronectin to enhance cell adhesion.
A: Optimize the choice and dilution of antibodies, extend incubation time, and ensure cell viability during processing and analysis.
A: Optimize cell lysis conditions, use appropriate immunoprecipitation reagents and antibodies for TNFRSF18 protein, ensure sufficient incubation time, and proper washing steps.
A: Optimize the design of sgRNA, improve the expression efficiency of Cas9, ensure the accessibility of the target sequence, and use efficient transfection methods.
A: Optimize protein extraction process, ensure suitable electrophoresis and transfer conditions, and use high-affinity and specificity antibodies for detection.
A: Use standardized apoptosis detection methods, ensure consistency in experimental conditions, including the concentration and duration of treatment agents, and perform multiple repetitions to increase the reliability of data.
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