Dog TNFRSF18 Stable Cell Line - HEK293T

Name: Dog TNFRSF18 Stable Cell Line - HEK293T
SKU: CSC-RO0563
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0563

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0563
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Dog TNFRSF18.
Target Gene	TNFRSF18
Gene Species	Canis lupus familiaris (Dog)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

How to improve cell adhesion in Dog TNFRSF18 Stable Cell Line - HEK293T during cell adhesion experiments?

A: Try changing the culture medium or coating culture plates with cell adhesion promoters such as collagen or fibronectin to enhance cell adhesion.

How to optimize the detection of TNFRSF18 expression in flow cytometry analysis of Dog TNFRSF18 Stable Cell Line - HEK293T?

A: Optimize the choice and dilution of antibodies, extend incubation time, and ensure cell viability during processing and analysis.

How to improve co-immunoprecipitation efficiency in protein interaction studies with Dog TNFRSF18 Stable Cell Line - HEK293T?

A: Optimize cell lysis conditions, use appropriate immunoprecipitation reagents and antibodies for TNFRSF18 protein, ensure sufficient incubation time, and proper washing steps.

How to enhance CRISPR/Cas9 gene editing efficiency in Dog TNFRSF18 Stable Cell Line - HEK293T?

A: Optimize the design of sgRNA, improve the expression efficiency of Cas9, ensure the accessibility of the target sequence, and use efficient transfection methods.

How to enhance the detection signal of TNFRSF18 protein in Western Blot experiments with Dog TNFRSF18 Stable Cell Line - HEK293T?

A: Optimize protein extraction process, ensure suitable electrophoresis and transfer conditions, and use high-affinity and specificity antibodies for detection.

How to ensure the accuracy and reproducibility of apoptosis experiments in Dog TNFRSF18 Stable Cell Line - HEK293T?

A: Use standardized apoptosis detection methods, ensure consistency in experimental conditions, including the concentration and duration of treatment agents, and perform multiple repetitions to increase the reliability of data.

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