Rat Tnfrsf18 Stable Cell Line - HEK293T

Name: Rat Tnfrsf18 Stable Cell Line - HEK293T
SKU: CSC-RO0564
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0564

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0564
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Rat Tnfrsf18.
Target Gene	Tnfrsf18
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tnfrsf18 tumor necrosis factor receptor superfamily, member 18 [ Mus musculus ]
Gene Symbol	Tnfrsf18
Synonyms	AITR; Gitr
Gene Description	tumor necrosis factor receptor superfamily, member 18
GeneID	21936
Uni ProtID	B1ASL4
mRNA Refseq	NM_021985.2
Protein Refseq	NP_068820.1
Chromosome Location	4; 4 E
Function	protein binding;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How to reduce the abnormally high cell death rate in apoptosis experiments with Rat Tnfrsf18 Stable Cell Line - HEK293T?

A: Check whether the culture conditions are appropriate, such as temperature and CO₂ concentration, optimize the culture medium formulation, and reduce physical or chemical stimuli during cell culture.

How to improve the efficiency of specific protein co-immunoprecipitation in Rat Tnfrsf18 Stable Cell Line - HEK293T?

A: Optimize lysis buffer and conditions to enhance the extraction efficiency of the target protein, and use high-specificity antibodies and appropriate immunoprecipitation reagents.

How to improve drug response sensitivity in drug screening experiments with Rat Tnfrsf18 Stable Cell Line - HEK293T?

A: Adjust the drug treatment concentration and duration to match the cell state, and optimize cell density and culture conditions if necessary.

How to enhance the detection signal of marker proteins in flow cytometry analysis of Rat Tnfrsf18 Stable Cell Line - HEK293T?

A: Optimize the choice of antibodies and incubation conditions, including antibody concentration and incubation time, and ensure cell viability during processing and analysis.

How to ensure the specificity and efficiency of gene silencing in Rat Tnfrsf18 Stable Cell Line - HEK293T?

A: Precisely design siRNA or shRNA targeting the Tnfrsf18 gene, optimize transfection conditions and silencing agent concentration, and conduct control experiments to ensure specificity.

How to ensure the accuracy and repeatability of cell proliferation experiments with Rat Tnfrsf18 Stable Cell Line - HEK293T?

A: Standardize culture conditions and experimental procedures, use accurate cell counting methods, and perform multiple repetitions to ensure the stability of data.

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