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Cat.No. : CSC-RO0564 Host Cell: HEK293T
| Cat. No. | CSC-RO0564 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Rat Tnfrsf18. |
| Gene | Tnfrsf18 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Check whether the culture conditions are appropriate, such as temperature and CO₂ concentration, optimize the culture medium formulation, and reduce physical or chemical stimuli during cell culture.
A: Optimize lysis buffer and conditions to enhance the extraction efficiency of the target protein, and use high-specificity antibodies and appropriate immunoprecipitation reagents.
A: Adjust the drug treatment concentration and duration to match the cell state, and optimize cell density and culture conditions if necessary.
A: Optimize the choice of antibodies and incubation conditions, including antibody concentration and incubation time, and ensure cell viability during processing and analysis.
A: Precisely design siRNA or shRNA targeting the Tnfrsf18 gene, optimize transfection conditions and silencing agent concentration, and conduct control experiments to ensure specificity.
A: Standardize culture conditions and experimental procedures, use accurate cell counting methods, and perform multiple repetitions to ensure the stability of data.
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