Panoply™ Human ERBB3 Knockdown Stable Cell Line

Here, researchers constructed a protein-based model to improve the prognostic predictive ability of head and neck squamous cell carcinoma (HNSCC). They downloaded proteomic data from the Cancer Proteome Atlas (TCPA) portal for the HNSCC cohort. The TCPA HNSCC cohort was randomly divided into a discovery cohort and a validation cohort. Researchers constructed a risk profile consisting of five proteins, which was strongly correlated with overall survival (OS) in the discovery cohort. Similar results were observed in the validation cohort. This protein-based risk profile was identified as an independent prognostic factor for HNSCC. The nomogram model constructed based on this protein risk profile showed good performance in predicting OS. Immunohistochemical (IHC) analysis showed that higher HER3_pY1289 staining intensity was associated with a worse prognosis for HNSCC. Inhibition of HER3 suppressed the proliferation and invasiveness of HNSCC cells. In conclusion, the researchers constructed a protein-based risk profile that can be used to accurately predict the prognosis of HNSCC, which might provide valuable information for optimal individualized treatment regimens.

In head and neck squamous cell carcinoma (HNSCC) cells, HER3 knockdown significantly reduced HER3 mRNA levels (Figure 1A). Western blotting results showed that the expression levels of HER3 and HER3_pY1289 were significantly lower in HER3 knockdown cells compared to the control group (Figure 1B). MTT assays showed that HER3 knockdown cells had lower OD values at different time points (48 h, 72 h, and 96 h) compared to the control group (Figure 1C). Similarly, the percentage of EdU-positive cells in HER3 knockdown HNSCC cells was also lower (Figure 1D-E). The number of HER3 knockdown cells that crossed the cell membrane was significantly less than that in the control group (Figure 1F, 1G).

Figure 1. Knockdown of HER3 suppresses the proliferation and invasion of HNSCC cells. (Zhao X, et al., 2020)