Cat.No. : CSC-RO0043 Host Cell: CHO-K1
| Cat. No. | CSC-RO0043 |
| Description | This cell line is a stably transfected cell line which expresses Human CD38 without any tag. |
| Gene | CD38 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | CD38 CD38 molecule [ Homo sapiens (human) ] |
| Gene Symbol | CD38 |
| Synonyms | ADPRC1; ADPRC 1 |
| Gene ID | 952 |
| UniProt ID | P28907 |
| Chromosome Location | 4p15.32 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CD38 Stable Cell Line-CHO originates from the cloning of the human CD38 gene, typically achieved by introducing human CD38 cDNA into CHO (Chinese Hamster Ovary) cells. These CHO cells are then screened to ensure stable expression of the CD38 gene. Once a stable cell line is successfully established, they are maintained and expanded periodically in culture medium to ensure continuous CD38 expression.
A: he Human CD38 Stable Cell Line-CHO has several research applications, including but not limited to: Drug screening: Used to test the potential effects of new drugs on CD38, particularly in the context of cancer therapy.
Immunology research: Employed to investigate the role of CD38 in immune cell function, such as lymphocyte activation and regulation.
Cancer research: Utilized to study CD38 expression in different cancer types and its relationship with tumor growth and treatment resistance.
Cell signaling: Applied to study the interaction of CD38 with cellular signaling pathways and its role in intracellular calcium regulation.
Immunotherapy: Utilized to develop CD38 as a target for immunotherapies, such as CAR-T cell therapy.
A: When using the Human CD38 Stable Cell Line-CHO for research, several experimental considerations should be kept in mind:
Cell culture conditions: CHO cells typically require specific culture conditions, including choice of medium, temperature, CO2 concentration, and culture duration.
CD38 expression stability: Ensuring stable expression of CD38 in the cell line is crucial. Regularly check CD38 expression levels to ensure experimental reproducibility.
Preventing cell contamination: Regularly check for contamination by bacteria, fungi, or other cells and take appropriate measures to prevent contamination.
Experimental design: Design experiments based on research questions and include appropriate control groups to ensure reliable experimental results.
Biosafety: Adhere to biosafety standards when working with CHO cells, especially when dealing with infectious diseases or pathogens.
Data analysis: Proper data analysis and statistical methods are crucial for obtaining accurate conclusions from experiments.
A: CD38 as a potential target for immunotherapy has garnered significant research interest. Specifically, CAR-T cell therapy has been employed in clinical trials to treat CD38-positive multiple myeloma patients, achieving some success. Additionally, anti-CD38 antibody drugs have received FDA approval for the treatment of multiple myeloma. Therefore, CD38 holds promising applications in immunotherapy and may play a crucial role in future cancer treatments.
A: CD38 is associated with various diseases and pathological processes, including:Leukemia and lymphoma: CD38 is overexpressed in multiple types of leukemia and lymphoma, making it a potential therapeutic target. Autoimmune diseases: CD38 may be involved in the pathogenesis of autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus.
A: CD38 is an enzyme located on the cell membrane, primarily expressed in immune cells and various tissues. Its main physiological roles include:Subcellular calcium regulation: CD38 regulates intracellular calcium ion concentration, participating in various cell signaling pathways, including intracellular calcium storage and release. Immune regulation: CD38 plays a significant role in modulating immune cell activation, differentiation, and immune response regulation, particularly in regulating lymphocyte function.
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