Cat.No. : CSC-RO1127 Host Cell: CHO-K1
| Cat. No. | CSC-RO1127 |
| Description | This cell line is engineered to stably express monkey CD38 in CHO-K1 cells. |
| Gene | CD38 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A growing number of patients with acute lymphoblastic leukemia (ALL) are being treated with antibody-based immunotherapy that targets CD38. The researchers used antibody-based immunotherapy targeting CD38 to treat patients with acute lymphoblastic leukemia (ALL). They examined the effectiveness of daratumumab (DARA), an IgG1 antibody, and its IgA2 variant (DARA-IgA2) in antibody-dependent cellular phagocytosis (ADCP) by macrophages and antibody-dependent cell-mediated cytotoxicity (ADCC) by polymorphonuclear cells (PMNs) against T-ALL cell lines and patient-derived tumor cells. They examined the effects of CD47-SIRPα interactions on T-ALL cell killing using CD47 and QPCTL knock-out cells and evaluated the effects of all-trans retinoic acid (ATRA) on improving ADCP and ADCC, particularly with DARA-IgA2.
Figure 1. The researchers utilized various cell lines including Human T-ALL cell lines HSB-2, MOLT-13, P12-ICHIKAWA, CHO-S, and CHO-K1 from DSMZ. They generated human CD38 transgenic CHO-K1 cells and performed CRISPR/Cas9 to generate MOLT-13 QPCTL KO and MOLT-13 CD47 KO cells using specific gRNA sequences. (Baumann N, et al., 2022)
A: Our monkey CD38 stable cell line undergoes rigorous purification and validation procedures to ensure its purity and stability. We use a variety of techniques to identify cell lines, including PCR, immunofluorescence staining, and Western blot. We also regularly perform cytological testing of cell lines to ensure they are free of hybridization or mutation.
A: Our monkey CD38 stable cell line expresses stable levels and is widely used in cell signaling research. We provide detailed experimental data support, including Western blot analysis, flow cytometry, etc. Customers can refer to these data to evaluate the performance of our products.
A: Our monkey CD38 stable cell lines have been widely used in drug screening experiments. We provide relevant literature support demonstrating the potential of our products in drug screening. Customers can refer to these documents to understand the performance and application range of our products.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I have recently used the Monkey CD38 Stable Cell Line - CHO-K1 and found it performs well in culture. The expression of CD38 molecules is not only stable, but also shows good results in both intracellular and extracellular functions. In particular, this cell line can efficiently synthesize and hydrolyze cADPR, effectively regulating intracellular calcium ion levels. In addition, the technical support provided by the supplier was very professional and solved all the problems I encountered during the experiment. The entire experience was very smooth and would be recommended to researchers with similar needs.
As a long-time biotech researcher, I am deeply impressed by the stable performance of Monkey CD38 Stable Cell Line - CHO-K1. This cell line showed high levels of protein expression during culture, especially the dual function of the CD38 molecule, which has both extracellular and intracellular effects. During use, cells grew well and gene expression was stable. The services of the platform merchants are also very good. Quick responses and professional suggestions made my research smoother.
When using Monkey CD38 Stable Cell Line - CHO-K1 for experiments, I felt the high quality and stability of this cell line. The CD38 molecule plays an obvious role both inside and outside cells, especially its ability to synthesize and hydrolyze cADPR, which greatly guarantees the reliability of experimental data. The high protein expression levels of this cell line are ideal for my research needs. At the same time, I am very satisfied with the professional service and timely support of the supply platform, which ensured the smooth progress of the experiment.
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