Human AKT1 Knockdown Cell Line-HeLa

Name: Human AKT1 Knockdown Cell Line-HeLa
SKU: CSC-RK0255
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0255

Host Cell : HeLa Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-RK0255
Target Gene	AKT1
Abbr	HeLa-HuAKT1 Knockdown
Alias	AKT1, AKT, PKB, PRKBA, RAC, MGC99656
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Morphology	Epithelial
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	AKT1 v-akt murine thymoma viral oncogene homolog 1 [ Homo sapiens ]
Gene Symbol	AKT1
Synonyms	AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA
Gene Description	v-akt murine thymoma viral oncogene homolog 1
GeneID	207
Uni ProtID	B0LPE5
mRNA Refseq	NM_001014431.1
Protein Refseq	NP_001014431.1
Chromosome Location	14q32.32
Function	ATP binding; ATP binding; enzyme binding; identical protein binding; kinase activity; nitric-oxide synthase regulator activity; phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein kinase C binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity;
Pathway	AKT phosphorylates targets in the cytosol, organism-specific biosystem; AKT phosphorylates targets in the nucleus, organism-specific biosystem; AKT-mediated inactivation of FOXO1A, organism-specific biosystem; AMPK signaling, organism-specific biosystem; Activation of BAD and translocation to mitochondria, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem;
MIM	164730

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The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

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