Cat.No. : AD00306Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00306Z |
| Description | Human Adenovirus Type5 (dE1/E3) expressing Akt (also known as Akt1) under a CMV promoter. No fusion tag, pre-made adenovirus, ready to ship and ready to use format. |
| Target Gene | AKT1 |
| Product Type | Adenoviral particle |
| Insert | Akt (also known as Akt1, PKB) |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | V-akt Murine Thymoma Viral Oncogene Homolog 1 |
| Gene Symbol | AKT1 |
| Synonyms | Akt1 |
| Gene ID | 207 |
| mRNA Refseq | M63167 |
Ferulic acid, a phenolic acid widely found in corn, wheat, and flax, has potent antitumor effects in various cancer cell lines. However, the antitumor effects of ferulic acid on osteosarcoma remain unclear. Here, the researchers examined the effects of ferulic acid on osteosarcoma cells and explored its potential mechanisms. MTT assay and Annexin V-FITC apoptosis assay showed that ferulic acid dose-dependently inhibited the proliferation of 143B and MG63 osteosarcoma cells and induced apoptosis. In addition, flow cytometry assay and Western blotting confirmed that ferulic acid induced G0/G1 phase arrest and downregulated the expression of cell cycle-related proteins CDK 2, CDK 4, and CDK 6. In addition, ferulic acid upregulated Bax, downregulated Bcl-2, and subsequently enhanced caspase-3 activity. More importantly, ferulic acid dose-dependently inhibited PI3K/Akt activation. The antiproliferative and pro-apoptotic effects of ferulic acid were reversed using adenovirus expressing active Akt. These results suggest that ferulic acid may inhibit proliferation and induce apoptosis by inhibiting the PI3K/Akt pathway in osteosarcoma cells. Ferulic acid is a new therapeutic drug for osteosarcoma.
To determine whether the PI3K/Akt pathway is involved in the antitumor effect of ferulic acid, the protein level of activated Akt (p-Akt) in human osteosarcoma cells was detected by IF and WB. As shown in Figures 1A and 1B, p-Akt in MG63 cells was significantly reduced after ferulic acid treatment in a dose-dependent manner. To further determine the effect of inhibition of the PI3K/Akt pathway on the antitumor effect of ferulic acid, cells were treated with LY294002, a specific inhibitor of PI3K. As shown in Figures 1C and 1D, LY294002 also had an antitumor effect, which was similar to ferulic acid. In addition, overexpression of active Akt in the adenovirus (Ad-AKT) system reversed the antitumor effect of ferulic acid, eliminating the inhibitory effect on cell proliferation and promoting apoptosis (Figures 1C and 1D).
Figure 1. Effect of ferulic acid on PI3K/AKT signal activation in osteosarcoma cells. (Wang T, et al., 2016)
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Achieved strong Akt overexpression with minimal cytotoxicity. Creative Biogene’s product was pivotal for our kinase pathway research.
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