Human AKT1 Knockout Cell Line-DLD-1

Name: Human AKT1 Knockout Cell Line-DLD-1
SKU: CSC-RT0017
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0017

Target Gene : AKT1 Host Cell : DLD-1

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0017
Description	DLD-1-AKT1 (-/-) is a cell line with a homozygous knockout of human AKT1
Target Gene	AKT1
Host Cell	DLD-1
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	AKT1 v-akt murine thymoma viral oncogene homolog 1 [ Homo sapiens ]
Gene Symbol	AKT1
Synonyms	AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA
Gene Description	v-akt murine thymoma viral oncogene homolog 1
GeneID	207
Uni ProtID	B0LPE5
mRNA Refseq	NM_001014431.1
Protein Refseq	NP_001014431.1
Chromosome Location	14q32.32
Function	ATP binding; ATP binding; enzyme binding; identical protein binding; kinase activity; nitric-oxide synthase regulator activity; phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein kinase C binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity;
Pathway	AKT phosphorylates targets in the cytosol, organism-specific biosystem; AKT phosphorylates targets in the nucleus, organism-specific biosystem; AKT-mediated inactivation of FOXO1A, organism-specific biosystem; AMPK signaling, organism-specific biosystem; Activation of BAD and translocation to mitochondria, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem;
MIM	164730

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Easy to knock out

The knockout and testing was done within 10 days and I really recommend this reagent, the efficiency is superb.

United States

2023-03-19

Costly

According to my previous method of experimentation, reagents, etc., this experiment would have taken me two months to complete, and I used this product for only one month, directly saving half the time.

United States

2023-02-18

Easy to use

Maybe it's a bit of metaphysics, I hadn't been able to get a positive result before, my teacher suggested I change the reagents from another company and I was very surprised when the result came back

United States

2022-12-16

Perfect results

The unsatisfactory results had caused me so much headache that I was tempted to give up on this part of the experiment until I used this reagent to produce good results and I realised what my problem was.

United States

2023-01-26

Costly

This reagent is almost as effective as a very expensive brand I have used before, but it is so cheap that it does seem to need more comparison.

United States

2023-02-25

Will continue to use

With the mentality of giving it a try, I bought a small-sized knockout reagent, and after trying it once, I decided to buy other specifications of reagent and continue to use it.

United States

2023-01-15

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Human AKT1 Knockout Cell Line-DLD-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Easy to knock out

Costly

Easy to use

Perfect results

Costly

Will continue to use

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