Vaccinia Capping System

Name: Vaccinia Capping System
SKU: RSNK-026

For research use only. Not intended for any clinical use.

Cat.No.

RSNK-026

Description

Based on the Vaccinia virus Capping Enzyme (VCE), the Vaccinia Capping System provides the necessary components to add 7-methylguanylate cap structures (m7Gppp5′N, Cap 0) to the 5′end of RNA.

Concentration

10 U/μl

Size/Form

500 U

Source

An E. coli strain that carries the Vaccinia capping enzyme gene.

Storage

Store at -20 °C

Publications

Q & A

Customer Reviews

Customer Q&As

What is Vaccinia Capping System?

A: The Vaccinia Capping System is a co-transcriptional process used by the Vaccinia virus, a type of poxvirus, to protect and stabilise its mRNA. During this process, the 5' end of the mRNA is modified, or "capped", and 3' end is polyadenylated.

What is the function of the vaccinia capping system?

A: This capping process is unique to viruses and some eukaryotes, and serves to increase the stability and translation efficiency of the viral mRNAs. It also helps the mRNA to evade the host's immune response.

What is the mechanism of mRNA capping?

A: The mRNA cap is a hypermethylation modification at the 5' end of RNA transcribed by RNA pol II. It protects RNA from degradation, recruits complexes involved in RNA processing, export and translation initiation, and marks cellular mRNA as "self" to avoid recognition by the innate immune system.

What are Cap-0 and Cap-1?

A: Cap-0 is a N7-methyl guanosine connected to the 5′ nucleotide through a 5′ to 5′ triphosphate linkage, typically refers to as m7G cap or m7Gppp- in the literature. In the cell, the Cap-0 structure is essential for the efficient translation of the mRNA that carries the cap. An additional methylation on the 2′O position of the initiating nucleotide generates Cap-1, or refers to as m7GpppNm-, where Nm denotes any nucleotide with a 2′O methylation. Cap-1 has been shown to be important in evading the cellular innate immune response in vivo.

Can Vaccinia Capping Enzyme use cap analogs in capping reaction?

A: No. Vaccinia capping enzymes use GTP and SAM to generate caps on 5' triphosphate RNAs, typically synthetic transcripts produced by in vitro transcription. On the other hand, cap analogs such as GpppG, m7GpppG, m7GpppA and anti-reverse cap analogs are used during in vitro transcription, where the cap analog is incorporated directly into the transcript as the first nucleotide at the 5' end.

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