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RSNK-012

Anti-Reverse Cap Analog (ARCA)

For research use only. Not intended for any clinical use.

Cat. No. :   RSNK-012

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Product Information

Cat. No. RSNK-012
Description Anti-Reverse Cap Analog (ARCA) is a cap analog used during in vitro transcription for the generation of capped transcripts. ARCA is a modified cap analog in which the 3' OH group (closer to m7G) is replaced with 3’O-Me. With this modification, ARCA can only insert in the proper orientation, which reduces the possibility of synthesizing non-translational mRNA, improving translation efficiency.
Alias Anti Reverse Cap Analog, 3´-O-Me-m7G(5')ppp(5')G
Applications Used to forming ARCA, 3´-O-Me-m7G(5')ppp(5')G
Molecular Formula C22H32N10O18P3
Molecular Weight 817.4
Size 1 µmol, 5 µmol
Purity ≥95%
Storage Store at -20°C.
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Customer Q&As
What is the effect of reverse transcription on in vitro transcription experiments?

A: Reverse transcription is a common problem during in vitro transcription experiments where high concentration of the reverse strand nucleotide can be mistakenly incorporated into the RNA by RNA polymerase. This can lead to the production of reverse-oriented mRNA molecules, which can interfere with downstream analyses.

What is the role of ARCA in vitro transcription experiments?

A: The incorporation of ARCA instead of traditional cap analogs can prevent the production of reverse-oriented mRNA molecules during transcription. By adding a 3' to 5' linkage, ARCA blocks the RNA polymerase from recognizing the reverse strand nucleotide as a template, eliminating the possibility of initiating transcription in the reverse direction.

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Customer Reviews
High Efficiency

ARCA can only insert in the correct orientation, resulting in mRNAs that are translated twice as efficiently. I like it.

United States

Effective tool

Great kit. The capping rate can reach approximately 80% and the crude transcript can reach 2-2.5 mg/ml.

United States

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