Cat.No. : LVG00002Z
Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL / ≥1*10^9 TU/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | LVG00002Z |
| Description | Lentivirus particles containing second generation of anti-CD19 CAR (chimeric antigen receptor) scFv-41BB-CD3zeta. |
| Target Gene | CD19 |
| Application | The scFv(CD19)-41BB-CD3zeta CAR-T lentivirus is intended for use in generating CAR-T (chimeric antigen receptor T cell) therapies that specifically target cells expressing CD19. Applications for research using the scFv(CD19)-41BB-CD3zeta CAR-T lentivirus include: Development of CAR-T therapies: Researchers can use this lentivirus to transduce (introduce) T cells to express a chimeric antigen receptor (CAR) that targets CD19. These modified T cells can be used to study the efficacy, mechanism, and optimization of CAR-T cell therapies. Cancer research: By targeting CD19, researchers can study the therapeutic potential and mechanism of CAR-T cells in treating B-cell malignancies. This may involve in vitro studies using cell lines or in vivo studies using animal models. Signal transduction studies: The construct includes costimulatory and activation domains that are critical in T cell signaling. Researchers can study how these domains affect T cell behavior, survival, and function. T cell biology: Understanding the activation, proliferation, and cytotoxic responses of T cells equipped with different CAR constructs can provide insights into T cell biology and immune responses. Optimization of CAR design: By varying components within the CAR (e.g., using different co-stimulatory domains), researchers can optimize CAR design to improve efficacy, durability, and safety for therapeutic applications. |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
| Gene Name | CD19 CD19 molecule [ Homo sapiens ] |
| Gene Symbol | CD19 |
| Synonyms | CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802; |
| Gene ID | 930 |
| UniProt ID | P15391 |
| mRNA Refseq | BC006338 |
| Chromosome Location | 16p11.2 |
| Function | receptor signaling protein activity; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; BCR signaling pathway, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; |
| MIM | 107265 |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Consistency in quality and performance is critical for our experiments. The scFv(CD19)-41BB-CD3zeta CAR-T Lentivirus consistently delivers reliable results, which has been instrumental in the success of our ongoing research.
As a principal investigator, I have tried multiple sources for CAR-T lentiviral vectors, but the transduction efficiency of the scFv(CD19)-41BB-CD3zeta lentivirus from Creative Biogene is unmatched.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
