Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
Harness the power of protein degraders for precise protein degradation, expanding druggable targets and enhancing therapeutic effectiveness for cutting-edge drug discovery.
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Stable expression over 15 generations with rapid cell line development in just 3 months. Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
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Cat. No. :
VET1290
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Product Information
Cat. No.
VET1290
Host Cell
plant, Escherichia coli
Promoter
CaMV 35S
Resistance
Kanamycin
Vector Type
Expression Vector
Vector Size
6382 bp
Quick Inquiry
Background
Case Study
Applications
Publications
Q & A
Customer Reviews
The pGreenII 0800-Luc vector is a versatile molecular tool that falls under the plant series classification of plasmids and is primarily used for protein overexpression in plant cells. It has a total size of 6382 base pairs. Its promoter sequences encompass both Cauliflower Mosaic Virus 35S (CaMV 35S) and T7, which are vital for the initiation of transcription in plants and bacteria respectively, hence ensuring the optimal activity of the vector in plant cells.
The pGreenII 0800-Luc vector includes two replicons, namely, pSa ori and ori. The presence of these replicons allows the plasmid to replicate upon integration into the host DNA, hence propagating the gene of interest in the host cell. This vector carries kanamycin resistance (Kan), allowing for the selection of successfully transformed plants. The encoding gene for Kanamycin resistance aids in the selection of transformed cells by conferring resistance to the antibiotic kanamycin, which would otherwise be lethal to the host cells.
Case Study 1
Enhancers located in introns are highly abundant and play an important role in the regulation of gene expression in mammalian species.In contrast,the functions of plant intronic enhancers are largely unexplored,and only a few plant intronic enhancers have been reported.Here,the researchers performed genome-wide predictions of intronic enhancers in Arabidopsis using DNase I sequencing-based open chromatin signatures.941 and 1,271 candidate intronic enhancers were identified with potential functions in seedling and floral tissues,respectively.Transgenic lines containing homozygous deletions of predicted intronic enhancers associated with two different genes were created using CRISPR/Cas-based genome editing.Using these lines,the researchers demonstrate that deletion of candidate enhancers results in varying levels of transcriptional repression of homologous genes at specific developmental stages.
In this study,the pGreenII 0800-LUC vector purchased from Creative Biogene was involved in the Transient expression assay.Luciferase reporter vectors with three copies of the predicted SPL9 binding motif ATATGTACTTTA or the EDT1 binding motif CATTAAATTA were made using pGreenII 0800-LUC(Figure 1C).The researchers examined the ability of SPL9 or EDT1 to activate reporter gene expression in Nicotiana benthamiana leaves.Transient expression assays showed that the ATTTGTACTTTA sequence was strongly activated by SPL9(Fig.1D),whereas the CATTAAATTA sequence was activated by EDT1 at a modest but significant level(Fig.1E).Collectively,these results indicate that the predicted SPL9-and EDT1-binding motifs identified in the DHS7#1 and DHS7#2 enhancers are functional.
Figure 1.Functional assays of the SPL-and EDT1-binding motifs identified in the DHS7#1 and DHS7#2 enhancers.(Meng F,et al.,2021)
Case Study 2
Transcript abundance and PA levels in the transgenic hairy roots of pinto bean.
Background
Many common bean varieties lose market value due to postharvest seed coat darkening caused by proanthocyanidin (PA) accumulation and oxidation. This preprint explores the molecular regulation beyond the P bHLH transcription factor in pinto beans. The authors identify that P acts with PvMYB3A and PvWD9 in a protein complex to regulate PA biosynthetic gene expression. While P does not bind DNA directly, it partners with PvMYB3A, which binds the PvANR2B promoter. PvWD9 is essential but requires additional plant factors for complex formation. The slow-darkening Psd isoform functions less efficiently, offering targets to improve seed coat traits. Key Findings
P alone is insufficient for PA biosynthesis in common bean hairy roots; however, P interacts with the MYB proteins PvMYB3A and PvMYB10B and the WD40 protein PvWD9 in the nucleus. Genetic complementation shows PvMYB3A and PvWD9 restore proanthocyanidin accumulation in Arabidopsis tt2 and ttg1 mutants, whereas PvMYB10B only partially complements. Yeast two-hybrid confirms P–PvMYB interactions, but PvWD9 association requires unknown plant-specific components. Among the tested transcription factors, PvMYB3A alone directly binds the PvANR2B promoter, while P activates anthocyanidin reductase expression indirectly through PvMYB3A. Co-expression or fusion of P with PvMYB3A strongly induces PvANR2B, PvDFR1A/B, PvMATE8 and PA accumulation in hairy roots; adding PvWD9 further enhances promoter activation ~636-fold. Replacing P with the slow-darkening isoform Psd reduces transactivation by ~14–17%. Thus, an MBW-like P–PvMYB3A–PvWD9 complex regulates pinto bean seed coat darkening. Contribution
Creative Biogene supplied the pGreenII-0800-LUC vector used for dual-luciferase promoter transactivation assays.
Case Study 3
Transcript abundance and PA levels in the transgenic hairy roots of pinto bean.
Background
This study investigates postharvest seed coat darkening in common bean (Phaseolus vulgaris), a trait reducing crop value due to proanthocyanidin (PA) oxidation. Beyond the major P bHLH gene, the authors identify PvMYB3A and PvWD9 as essential partners forming a transcriptional complex. P is not a DNA-binding protein; it interacts with PvMYB3A, which directly binds the PvANR2B promoter, while PvWD9 requires additional plant factor(s). The slow-darkening Psd isoform functions less efficiently. These findings clarify PA regulation and offer molecular targets for breeding beans with improved seed coat color stability. Key Findings
Key findings show that P alone is insufficient for proanthocyanidin (PA) biosynthesis in pinto bean. Using BiFC, Y2H, Y1H, dual-luciferase, hairy-root transformation, and Arabidopsis complementation, the study reveals a functional MBW-like complex comprising P/Psd, PvMYB3A, and PvWD9. P interacts with PvMYB3A and PvMYB10B, but not with PvWD9 in yeast, suggesting additional plant factor(s) are required for PvWD9 incorporation. PvMYB3A directly binds the PvANR2B promoter, whereas P and PvMYB10B do not, despite activating PvANR2B expression when combined with PvMYB3A. P-PvMYB3A fusion yields the highest PvANR2B activation and PA accumulation in hairy roots. PvWD9 further enhances promoter activation. PvMYB3A and PvWD9 complement Arabidopsis tt2 and ttg1 mutants, restoring PA accumulation. The Psd isoform regulates PA similarly but with lower efficiency. These results redefine the P gene's role, showing it is a non-DNA-binding transcriptional cofactor, and identify PvMYB3A/PvWD9 as key regulators of postharvest seed coat darkening. Contribution
Creative Biogene provided the pGreenII-0800-LUC vector used to clone the PvUANR2B promoter for dual-luciferase assays. This vector enabled quantitative transactivation analysis, demonstrating that P, PvMYB3A, and PvWD9 combinations activate the PvANR2B promoter and that PvMYB3A is the direct DNA-binding component.
Case Study 4
Establishment of GRNs in each cross-species coexpression network and experimental validation of predicted regulatory relationships between TFs and CSLF6 genes
Background
This study introduces NEEDLE, a user-friendly network-enabled pipeline for gene discovery in non-model plant species. It integrates coexpression network modeling, gene regulatory network inference, cistrome analysis, and rapid in planta dual-luciferase validation. NEEDLE was validated using maize unfolded protein response and soybean seed development datasets, then applied to identify transcription factors regulating CSLF6 in Brachypodium and sorghum. The approach reveals conserved and divergent regulatory elements among grasses and enables prediction of biologically relevant upstream regulators from dynamic transcriptome data alone. Key Findings
NEEDLE successfully predicted upstream transcription factors by combining WGCNA coexpression modules with GENIE3 regulatory network inference, followed by promoter motif analysis and dual-luciferase validation. In maize, ZmbZIP60 ranked as the top regulator of the unfolded protein response; in soybean seed development, GmbZIP67 was top-ranked, with 55% of its predicted targets overlapping ChIP-seq data. Applied to CSLF6, NEEDLE identified BdbHLH62 and BdKNAT7 for BdCSLF6, and SbbHLH63 and SbNST1 for SbCSLF6. Dual-LUC assays confirmed BdKNAT7, BdbHLH62, and SbNST1 activate CSLF6 promoters, revealing cross-species regulatory conservation and divergence. The pipeline requires only dynamic transcriptome data and provides a practical framework for gene discovery in non-model crops. Contribution
Creative Biogene supplied the pGreenII 62-SK (VET1291) and pGreenII 0800-LUC (VET1290) vectors used to construct effector and promoter-reporter plasmids in this study. These reagents enabled the dual-luciferase assays that experimentally validated predicted transcription factor regulation of CSLF6 genes in planta.
The pGreenII 0800-LUC vector is a type of binary plasmid vector most commonly utilized in plant transformation studies. Here are a number of its primary applications:
Gene Expression Studies: pGreenII 0800-LUC can be utilized to express specific genes within plant cells. This can help to understand the role of individual genes in plant development and behavior.
Reporter Gene Assays: The vector contains the reporter gene Luciferase (LUC) which is used for monitoring the gene expression. The Luciferase enzyme generates light when it reacts with its substrate, luciferin. This light production is often measured to detect the activity of the gene of interest.
Studies involving the Agrobacterium tumefaciens-mediated plant transformation: Agrobacterium tumefaciens is a species of bacteria that uses a type of plasmid, a DNA molecule separate from chromosomal DNA, to insert its genetic material into plant cells. The pGreenII 0800-LUC vector can be introduced into this bacterium to facilitate the transformation process.
Gene Promoter Analysis: The pGreenII 0800-LUC vector can be used to carry a gene along with its promoter sequence (a region of DNA that initiates transcription of a particular gene) into a plant cell. Researchers can then assess how the promoter influences the expression of the gene of interest.
Investigation of Gene Function: The pGreenII 0800-LUC vector also allows for the investigation of gene function. This is achieved by altering gene sequences and observing the resulting effect on the plant.
Publications
Meng F, Zhao H, Zhu B, Zhang T, Yang M, Li Y, Han Y, Jiang J. Genomic editing of intronic enhancers unveils their role in fine-tuning tissue-specific gene expression in Arabidopsis thaliana. Plant Cell. 2021 Jul 19;33(6):1997-2014.
Customer Q&As
How are pGreenII 0800-Luc vectors different from conventional binary vectors?
A: pGreenII 0800-Luc vectors are different from conventional binary vectors because they have removed elements necessary for stable transformation, such as transformation selection genes, making them ideally suited for transient gene expression.
What is the size of the pGreenII 0800-Luc plasmid?
A: The size of the pGreenII 0800-Luc plasmid is 6382 bp.
What is the expression host for pGreenII 0800-Luc vectors?
A: The expression host for pGreenII 0800-Luc vectors is plant cells.
What are the sequencing primers used for the 5' end of pGreenII 0800-Luc vectors?
A: The sequencing primer for the 5' end of pGreenII 0800-Luc vectors is 35S:GACGCACAATCCCACTATCC.
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Customer Reviews
Useful
We first constructed the cDNA encoding the transcription factors onto the pUC 18-3HA vector, and then constructed the specific gene promoter sequence onto the pGreenII0800-LUC vector, which was very useful,more positive clones were observed.
United States
Stability
The pGreenII 0800-LUC vector also retains its usability after multiple freeze-thaw cycles, which is ideal for long-term research.
United Kingdom
Reliable esults
The pGreenII 0800-LUC vector has not only greatly facilitated my work, but also helped me to generate reliable and reproducible results.
United States
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Figure 1.Functional assays of the SPL-and EDT1-binding motifs identified in the DHS7#1 and DHS7#2 enhancers.(Meng F,et al.,2021)
Transcript abundance and PA levels in the transgenic hairy roots of pinto bean.
