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pAc5.1 V5-His B

For research use only. Not intended for any clinical use.
Cat.No.
VET1240
Promoter
P-AC5 (Actin 5C)
Selection
Ampicillin
Source
Insect cell, Escherichia coli
Species
Bacterial
Tag
V5 Epitope Tag , His Tag

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Q & A

Customer Reviews

Customer Q&As
What kind of promoter does the pAc5.1 V5-His B vector provide?

A: The pAc5.1 V5-His B vector offers the strong, constitutive promoter from the Drosophila actin 5C gene.

How does the pAc5.1 V5-His B vector improve the cloning efficiency?

A: The pAc5.1 V5-His B vector size is small, so it can improve DNA yield and subcloning efficiency.

How to simply purify the recombinant fusion protein by using this pAc5.1 V5-His B vector?

A: The pAc5.1 V5-His B vector has a 6 xHis tag at the c-terminus, which can be used to simply purify the recombinant fusion protein.

Can the pAc5.1 V5-His B vector achieve the effect of detecting the target protein quickly?

A: Because of the V5 epitope tag at the c-terminal end of the vector, this can be exploited for rapid detection of the immune hormone anti-V5 antibodies.

What elements are provided by the pAc5.1 V5-His B vector to facilitate cloning?

A: To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame

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Customer Reviews
Improve efficiency

The vector has small size,which is helpful for improving DNA yields and increase subcloning efficiency.

United States

10/09/2021

Rapid detection

C-terminal V5 epitope tag of this pAc5.1 V5-His B vector can be used for rapid detection with Invitrogens Anti-V5 Antibody.

United States

01/27/2022

Simple purification

pAc5.1 V5-His B vector has C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin.

United States

07/19/2022

Fast cloning

pAc5.1 V5-His BA set of three vectors-A, B, and C-is provided to facilitate cloning.

United States

11/03/2022

Multiple cloning sites

The pAc5.1 V5-His B vector has multiple cloning sites in a different reading frame, which contributes to gene cloning and protein expression.

United States

02/23/2023

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