Cat.No. : CSC-RO0532 Host Cell: HEK293T
| Cat. No. | CSC-RO0532 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Cyno DLL3. |
| Gene | DLL3 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Advanced small cell/neuroendocrine prostate cancer (SCNC) is always fatal. It can appear as treatment-emergent SCNC (t-SCNC) or arise from scratch following a protracted course of androgen restriction therapy. Researchers highlight delta-like ligand 3 (DLL3) in both kinds of SCNC using metastatic biopsies from patients with advanced cancer, and they find a correlation between DLL3 expression and a lower chance of survival. They create a PET agent, [89Zr]-DFO-DLL3-scFv, for the detection of DLL3 in mouse SCNC models. AMG 757 (tarlatamab), a prolonged half-life bispecific T cell engager, exhibits strong anti-tumor activity in patient-derived xenografts by efficiently targeting and eliminating DLL3-expressing cells. On the other hand, decreased DLL3 levels in late relapsing tumors after AMG 757 treatment point to antigen loss as a resistance mechanism, particularly in tumors with variable DLL3 expression. These results support a current clinical trial of AMG 757 in t-SCNC and de novo, demonstrating promising responses in confirmed SCNC cases.
Figure 1. The researchers cloned DLL3 cDNA into a lentiviral vector with a V5 epitope tag for detection. To produce a PET tracer for imaging experiments, the researchers used a single-chain variable fragment (scFv) that bounds to DLL3. They produced [89Zr]DFO-DLL3 by conjugating the scFv with deferoxamine (DFO) and chelating 89Zr. This showed cross-reactivity to DLL3 in humans, mice, and cynomolgus monkeys, but not to DLL1 or DLL4. (Chou J, et al., 2023)
A: This cell line, by stably expressing the DLL3 gene, provides a controllable experimental model for studying the role of the Notch signaling pathway in cell differentiation, proliferation, and development.
A: By observing the effects of different compounds on DLL3 expression and function, potential drugs that modulate the Notch signaling pathway can be screened, which is crucial for developing new therapies for related diseases.
A: As DLL3 plays a key role in the development of the nervous system, this cell line can be used to model the developmental processes of neural cells and study the molecular mechanisms of neurodegenerative diseases.
A: Genetic stability can be monitored by regularly conducting genetic tests, such as Southern blot or PCR, to track the copy number and expression levels of the DLL3 gene in the cell line.
A: This cell line can be used to study the role of DLL3 in tumor initiation, progression, and metastasis, as well as to evaluate cancer treatment strategies targeting DLL3.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Monkey DLL3 Stable Cell Line - HEK293T can efficiently and stably express the target gene, which is crucial for studying the function of the DLL3 protein and conducting drug screening.
This cell line is amenable to genetic engineering, facilitating various genetic experiments such as knockout, knock-in, or mutation analysis.
The Monkey DLL3 Stable Cell Line - HEK293T can be used in a variety of biological and medical research, including studies on neurodevelopment and cancer.
The cell line has excellent genetic stability, ensuring the stable expression of the target gene over long-term culture.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
