Human DLL3 Stable Cell Line - HEK293

Name: Human DLL3 Stable Cell Line - HEK293
SKU: CSC-SC004350-H1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC004350-H1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC004350-H1
Description	This cell line is engineered to stably overexpress human delta like canonical Notch ligand 3 (DLL3).
Target Gene	DLL3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DLL3 delta-like 3 (Drosophila) [ Homo sapiens ]
Gene Symbol	DLL3
Synonyms	SCDO1
GeneID	10683
Uni ProtID	Q9NYJ7
mRNA Refseq	NM_016941.3
Protein Refseq	NP_058637.1
Chromosome Location	19q13
Function	Notch binding;
Pathway	Neural Crest Differentiation, organism-specific biosystem; Notch Signaling Pathway, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch signaling pathway, conserved biosystem;
MIM	602768

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Background

Case Study

Applications

Q & A

Customer Reviews

The Human DLL3 Stable Cell Line, based on the HEK293 cell line, is designed to express the DLL3 (Delta-like ligand 3) gene. DLL3 is a member of the Notch signaling pathway, which is essential for cell fate determination and differentiation during development. It is particularly important in the nervous system and has been linked to neurodegenerative diseases. The HEK293 cell line, derived from human embryonic kidney cells, is chosen for its high transfection efficiency and ability to support stable expression of foreign genes. This stable cell line is a valuable resource for studying the role of DLL3 in various biological processes and for the development of therapeutic strategies targeting DLL3-mediated pathways.

The DLL3 Stable Cell Line - HEK293 cells are used by the researchers to study the processes of Notch signaling in the segmentation clock during mammalian development. Studying the oscillatory activity necessary for somitogenesis is made possible by this cell line. They investigate the genetic relationships between Lfng and Dll3, concentrating on their involvement in Notch activity modulation, using HEK293 cells that express DLL3. Studies show that when DLL3 is expressed along with LFNG, it can increase the activity of signaling in cells that also express DLL1 and NOTCH1. This model reveals the mechanism by which glycosylation and cis-inhibition regulate oscillatory Notch activation, which is necessary for the correct operation of the segmentation clock.

Figure 1 depicts the experimental results of the DLL3 protein with the EGF repeat region of the DLL1 protein as a target for LFNG extension. (doi: 10.1016/j.ydbio.2022.04.004) Figure 1. The researchers used DLL3 Stable Cell Line - HEK293 to study EGF repeats' glycosylation. Immunoblotting and mass spectrometry revealed LFNG's role in extending O-fucosylation, impacting DLL3 glycosylation profiles. (Bochter MS, et al., 2022)

The Human DLL3 Stable Cell Line - HEK293 is a model for studying the role of DLL3 in developmental processes and diseases. Its applications encompass:

(1) Neural Development Studies: DLL3 is involved in neural development. This cell line can be used to investigate the role of DLL3 in the formation of neural circuits and the differentiation of neural cells, which is crucial for understanding brain development and related disorders.

(2) Cancer Research: DLL3's expression in certain cancers makes this cell line a valuable tool for studying its role in tumorigenesis and for developing targeted therapies that could exploit DLL3's function in cancer cells.

(3) Drug Safety and Toxicity: The HEK293 cell line is widely used for drug testing. The stable expression of DLL3 in this cell line allows for the assessment of drug candidates' impact on neural-related pathways, which is important for evaluating potential side effects on the nervous system.

Customer Q&As

What is the role of the Human DLL3 Stable Cell Line - HEK293 in studying the Notch signaling pathway?

A: This cell line, by stably expressing DLL3, provides a controllable experimental model for studying the role of the Notch signaling pathway in cell differentiation, proliferation, and development.

How can the Human DLL3 Stable Cell Line - HEK293 be used for drug screening?

A: By observing the effects of different compounds on DLL3 expression and function, potential drugs that modulate the Notch signaling pathway can be screened, which is crucial for developing new therapies for related diseases.

What applications does the Human DLL3 Stable Cell Line - HEK293 have in neuroscience research?

A: As DLL3 plays a key role in the development of the nervous system, this cell line can be used to model the developmental processes of neural cells and study the molecular mechanisms of neurodegenerative diseases.

How to perform gene knockout or knock-in experiments in the Human DLL3 Stable Cell Line - HEK293?

A: Gene editing technologies such as CRISPR-Cas9 can be utilized to precisely knockout or knock-in specific genes in this cell line to study their effects on DLL3 function.

What applications does the Human DLL3 Stable Cell Line - HEK293 have in stem cell research?

A: This cell line can be used to study the role of DLL3 in maintaining stem cell states and promoting their differentiation, which is significant for regenerative medicine and stem cell therapies.

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Customer Reviews

Efficient Gene Expression

The Human DLL3 Stable Cell Line - HEK293 takes advantage of the high transfection efficiency of HEK293 cells to ensure efficient expression of the DLL3 gene, making experimental results more reliable.

Germany

2020-07-04

Guaranteed Stable Expression

Through selective resistance marking, the Human DLL3 Stable Cell Line - HEK293 ensures stable and long-term expression of DLL3 protein, improving the reproducibility of experiments.

United States

2022-08-03

Facilitates Tumor Research

Using the Human DLL3 Stable Cell Line - HEK293 allows for deeper investigation into the role of DLL3 in tumor initiation and progression, accelerating the discovery and validation of tumor treatment targets.

United States

2020-11-05

Specific Expression of DLL3

The Human DLL3 Stable Cell Line - HEK293 specifically expresses the DLL3 protein in HEK293 cells through stable transfection, providing a powerful tool for studying the biological functions and mechanisms of DLL3.

United States

2020-12-24

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Human DLL3 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What is the role of the Human DLL3 Stable Cell Line - HEK293 in studying the Notch signaling pathway?

How can the Human DLL3 Stable Cell Line - HEK293 be used for drug screening?

What applications does the Human DLL3 Stable Cell Line - HEK293 have in neuroscience research?

How to perform gene knockout or knock-in experiments in the Human DLL3 Stable Cell Line - HEK293?

What applications does the Human DLL3 Stable Cell Line - HEK293 have in stem cell research?

Efficient Gene Expression

Guaranteed Stable Expression

Facilitates Tumor Research

Specific Expression of DLL3

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