Cat.No. : CSC-SC004350-H1 Host Cell: HEK293
| Cat. No. | CSC-SC004350-H1 |
| Description | This cell line is engineered to stably overexpress human delta like canonical Notch ligand 3 (DLL3). |
| Gene | DLL3 |
| Host Cell | HEK293 |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The DLL3 Stable Cell Line - HEK293 cells are used by the researchers to study the processes of Notch signaling in the segmentation clock during mammalian development. Studying the oscillatory activity necessary for somitogenesis is made possible by this cell line. They investigate the genetic relationships between Lfng and Dll3, concentrating on their involvement in Notch activity modulation, using HEK293 cells that express DLL3. Studies show that when DLL3 is expressed along with LFNG, it can increase the activity of signaling in cells that also express DLL1 and NOTCH1. This model reveals the mechanism by which glycosylation and cis-inhibition regulate oscillatory Notch activation, which is necessary for the correct operation of the segmentation clock.
Figure 1. The researchers used DLL3 Stable Cell Line - HEK293 to study EGF repeats' glycosylation. Immunoblotting and mass spectrometry revealed LFNG's role in extending O-fucosylation, impacting DLL3 glycosylation profiles. (Bochter MS, et al., 2022)
A: This cell line, by stably expressing DLL3, provides a controllable experimental model for studying the role of the Notch signaling pathway in cell differentiation, proliferation, and development.
A: By observing the effects of different compounds on DLL3 expression and function, potential drugs that modulate the Notch signaling pathway can be screened, which is crucial for developing new therapies for related diseases.
A: As DLL3 plays a key role in the development of the nervous system, this cell line can be used to model the developmental processes of neural cells and study the molecular mechanisms of neurodegenerative diseases.
A: Gene editing technologies such as CRISPR-Cas9 can be utilized to precisely knockout or knock-in specific genes in this cell line to study their effects on DLL3 function.
A: This cell line can be used to study the role of DLL3 in maintaining stem cell states and promoting their differentiation, which is significant for regenerative medicine and stem cell therapies.
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The Human DLL3 Stable Cell Line - HEK293 takes advantage of the high transfection efficiency of HEK293 cells to ensure efficient expression of the DLL3 gene, making experimental results more reliable.
Through selective resistance marking, the Human DLL3 Stable Cell Line - HEK293 ensures stable and long-term expression of DLL3 protein, improving the reproducibility of experiments.
Using the Human DLL3 Stable Cell Line - HEK293 allows for deeper investigation into the role of DLL3 in tumor initiation and progression, accelerating the discovery and validation of tumor treatment targets.
The Human DLL3 Stable Cell Line - HEK293 specifically expresses the DLL3 protein in HEK293 cells through stable transfection, providing a powerful tool for studying the biological functions and mechanisms of DLL3.
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