Cat.No. : CSC-RO0602 Host Cell: CT26
| Cat. No. | CSC-RO0602 |
| Description | This cell line is derived from CT26 and is engineered to stably overexpress Human DLL3. |
| Gene | DLL3 |
| Host Cell | CT26 |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The objective of the study was to examine the functional equivalency of Notch ligands DLL1 and DLL3, which are coexpressed in the mouse embryonic presomitic mesoderm. Different abnormalities are caused by mutations in DLL1 and DLL3, even though they co-express. In order to investigate this, DLL1 was swapped out with DLL3 in mice, which showed that DLL3 does not make up for DLL1. In Drosophila wing discs, DLL1 stimulates Notch whereas DLL3 does not. In neither Drosophila nor mice was there any evidence of antagonistic or repressive interactions between DLL1 and DLL3. Their biochemical nonequivalence is partly explained by variations in particular domains of DLL1 and DLL3, according to in vitro investigations. While DLL3 is mostly found in the Golgi apparatus, endogenous DLL1 is present on the surface of presomitic mesoderm cells.
Figure 1. To examine surface expression using Western blotting, immunoprecipitation, and biotinylation, the researchers created DLL3 stable cell lines. (Geffers I, et al., 2007)
A: This cell line has been verified through multiple passages, and DLL3 expression remains stable. We conducted experiments for 50 consecutive passages, and the expression level of DLL3 was quantitatively analyzed by Western Blot and flow cytometry. The results showed that there was no significant change in the expression of DLL3.
A: DLL3 expression was verified by Western Blot and flow cytometry. Western Blot results showed that in each passage, the DLL3 protein band was clear and the expression level was consistent. Flow cytometry analysis showed that the proportion of DLL3-positive cells was stable at more than 90%. We can provide detailed experimental data and maps for reference.
A: Human DLL3 Stable Cell Line-CT26 is suitable for DLL3-related signaling pathway research, antibody drug screening, immunological research, etc. Functional verification included the role of DLL3 in inhibiting the Notch signaling pathway. We verified the function of DLL3 through the Notch signaling pathway reporter gene system.
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We were able to learn a lot about tumor biology thanks to this cell line. Our research shed light on DLL3's function in SCLC. Similar to trying to find a needle in a haystack! In addition, the staff at Creative Biogene gave us outstanding assistance all through our research process.
The utilization of the Human DLL3 Stable Cell Line - CT26 revolutionized our drug screening procedures. We found some interesting drugs that might target DLL3 in neuroendocrine tumors. It's similar to winning the lottery in a scientific experiment! Strongly suggest it to anyone working in the industry.
Creative Biogene's DLL3 cell line is excellent. Our research on Notch signaling pathways in tumor biology has greatly benefited from it. They are a trusted source in the research community because of their expertise and high-caliber products.
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