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mCherry MMLV Retrovirus

mCherry MMLV Retrovirus

Cat.No. :  RV00003Z

Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL Size: 100 ul/500 ul/1 mL

Storage:  -80℃ Shipping:  Frozen on dry ice

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Lentivirus Particle Information

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Cat. No. RV00003Z
Description Premade MMLV-based, VSV-G pseudotyped retroviral particles that contain mCherry reporter gene.
Target Gene mCherry
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL or ≥1*10^8 TU/mL.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Creative Biogene ensures high-quality retrovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between retrovirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that retrovirus products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared retrovirus vectors to ensure they meet quality standards.
Sterility The retrovirus samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the retrovirus products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of retrovirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene retrovirus vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the retrovirus vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected retrovirus insert.
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High transduction efficiency

The mCherry MMLV Retrovirus gave us robust, red fluorescence across multiple cell types. The viral prep was clean, and transduction was efficient—ideal for tracking cell lineages.

United States

05/04/2021

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