CMV-mCherry AAV (Serotype PHP.eB)

The CMV-mCherry AAV (Serotype PHP.eB) vector is a recombinant adeno-associated virus (AAV) utilizing the neurotropic PHP.eB serotype for its capsid protein. Compared to traditional AAV serotypes, this serotype exhibits superior central nervous system (CNS) targeting and enhanced blood-brain barrier penetration. The vector carries the mCherry fluorescent reporter gene driven by the ubiquitously expressed cytomegalovirus (CMV) promoter, ensuring stable and sustained transgene expression in various cell types. Its main advantages include: 1) High transduction efficiency in both neurons and glial cells (more than 10 times higher than AAV9 in mouse models); 2) Low immunogenicity due to the non-pathogenic nature of AAV; 3) Long-term (months to years) transgene expression from episomal DNA without the risk of genomic integration; and 4) Scalable production.

This vector is widely used in neuroscience research for neural circuit mapping and cell phenotyping. The bright mCherry fluorescence, combined with techniques such as confocal microscopy or tissue clearing imaging (e.g., CLARITY), allows for high-resolution visualization of neuronal morphology. Its CNS targeting properties make it an ideal tool for studying neurodegenerative diseases (Alzheimer''s disease, Parkinson''s disease), allowing researchers to track transduced cells in animal models after systemic administration. Furthermore, the strong activity of the CMV promoter facilitates quantitative assessment of transduction efficiency in organs during biodistribution studies. In optogenetics experiments, mCherry can serve as an anatomical marker when co-expressed with light-sensitive opsins.