CAG-mCherry AAV (Serotype AAV-BI30)

scAAV-CMV-GFP (retrograde serotype) is a highly efficient viral vector system that combines the advantages of single-stranded adeno-associated virus (scAAV) with the specificity of a retrograde serotype. This modified vector features a self-complementary AAV genome (scAAV), eliminating the need for second-strand synthesis in target cells and enabling faster and stronger transgene expression compared to traditional single-stranded AAV vectors. The CMV (cytomegalovirus) promoter drives strong and widespread expression of the GFP reporter gene, allowing for clear visualization of transduced cells. The retrograde serotype capsid confers unique axonal transport properties, enabling efficient transduction of projecting neurons via retrograde transport to axon terminals—a key characteristic for studying neural circuits.

This AAV vector with retrograde transport capabilities has become an indispensable tool for mapping neural connections and manipulating specific neuronal populations. Researchers primarily use scAAV-CMV-GFP (retrograde serotype) for anterograde and retrograde tracing studies, where GFP expression reveals detailed projection patterns of labeled neurons. When combined with optogenetic tools or calcium indicators, this vector enables functional studies, allowing for the investigation of both the structure and function of neural circuits. Its retrograde capability allows for targeted delivery of the vector to neurons projecting to specific brain regions, enabling circuit-specific manipulations without direct injection into the cell body. Other applications include long-term tracking of neuronal morphology, monitoring of axonal transport dynamics, and studying neurodegenerative disease models with impaired retrograde transport. The GFP reporter gene allows for long-term, non-destructive visualization of infected cells, enabling longitudinal studies of neuronal development, plasticity, and degeneration.