Cat.No. : CSC-RO0662 Host Cell: VeroE6
| Cat. No. | CSC-RO0662 |
| Description | This cell line is engineered to stably overexpress human angiotensin I converting enzyme 2 (ACE2) in VeroE6 cells. |
| Gene | TMPRSS2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | VeroE6 |
| Host Cell Species | Chlorocebus sabaeus (Green monkey) |
| Stability | Validated for at least 10 passages |
| Application | 1. Study the mechanisms of viral entry and replication 2. Research and in vitro screening of potential drugs 3. Vaccine development 4. The production of pseudotyped viruses |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Human coronaviruses (HCoVs) are responsible for causing the common cold in humans. Four main types of HCoVs have been identified: HCoV-229E and HCoV-NL63 belong to the Alphacoronavirus genus, while HCoV-OC43 and HCoV-HKU1 belong to the Betacoronavirus genus. The researchers constructed a TCID50 assay for HCoV-OC43 based on CPE evaluation. Since HCoV-OC43 uses cell surface transmembrane serine protease 2 (TMPRSS2) to enter cells, VeroE6 cells expressing TMPRSS2 can show obvious CPE after infection with HCoV-OC43. The study found that VeroE6/TMPRSS2 cells showed obvious CPE 3 to 4 days after low-titer HCoV-OC43 infection. By comparing the CPE evaluation method with the IPA method, the researchers found that the titer values measured by the CPE evaluation method using VeroE6/TMPRSS2 cells 4 to 5 days after infection were less different from the reference titer values. Therefore, the TCID50 assay based on CPE evaluation of VeroE6/TMPRSS2 cells provides accurate titer values for future HCoV-OC43 studies.
Figure 1. Cell cytopathic effects (CPE) after high-titer HCoV-OC43 infection was assessed by the researchers. Using an Olympus IX71 inverted light microscope, HCT-8, VeroE6, and VeroE6/TMPRSS2 cells were infected with 2.0 × 10^5 TCID50/ml virus and their CPE was observed for 1 to 5 days after the infection. The cells were plated in 96-well plates. Following inoculation, VeroE6/TMPRSS2 cells were grown in DMEM with different amounts of fetal bovine serum. (Hirose R, et al., 2021)
A: Specific challenges in using the Human TMPRSS2 Stable Cell Line - VeroE6 to evaluate potential antiviral compounds include ensuring that the compounds directly target the activity of the TMPRSS2 enzyme rather than other cellular factors. Additionally, given the role of TMPRSS2 in the invasion of various viruses, distinguishing the inhibitory effects of compounds on different viruses presents a challenge. Finally, assessing the toxicity and in vivo efficacy of compounds requires complex in vitro and in vivo models, which can increase the complexity and cost of research.
A: The potential value of the Human TMPRSS2 Stable Cell Line - VeroE6 lies in its ability to simulate the process of viral entry into human cells via TMPRSS2, providing a powerful tool for researching and developing targeted therapeutic strategies against coronaviruses. By using this cell line, researchers can identify and evaluate compounds that block the activity of TMPRSS2, thereby preventing viral invasion and replication. This has significant implications for the development of new antiviral drugs and vaccines, especially against emerging or mutating strains of coronaviruses.
A: The Human TMPRSS2 Stable Cell Line - VeroE6, by providing a system that stably expresses the human TMPRSS2 enzyme, enables researchers to study in detail how SARS-CoV-2 utilizes this enzyme to facilitate the activation of its spike protein and subsequent fusion with the host cell membrane. This cell line allows scientists to observe the interactions between the virus and host cells at the molecular level, including how the virus circumvents the host's immune defense mechanisms. This is crucial for revealing the intricate mechanisms of viral invasion and developing strategies to block these interactions.
A: The application of the Human TMPRSS2 Stable Cell Line - VeroE6 in assessing viral transmission dynamics includes using this cell line as a model to study how viruses spread between cells, especially given the importance of TMPRSS2 in viral membrane fusion and entry processes. By analyzing the replication and spread patterns of the virus in this cell line, researchers can gain a better understanding of how the virus disseminates within the host, providing critical insights into viral transmission studies.
A: The Human TMPRSS2 Stable Cell Line - VeroE6 is not only crucial for viral research but also offers a unique perspective for understanding the role of TMPRSS2 in non-viral diseases. Since TMPRSS2 is involved in various biological processes and disease mechanisms, such as cancer development and inflammatory responses, using this cell line can reveal the biological functions of TMPRSS2 and its role in disease pathogenesis. This aids in discovering new disease biomarkers and therapeutic targets, expanding the scope of TMPRSS2 research applications.
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Through stable expression of TMPRSS2, its potential role in cellular signaling can be investigated.
This cell line aids in the screening and development of antiviral drugs targeting TMPRSS2, especially in the context of coronaviruses.
The Human TMPRSS2 Stable Cell Line - VeroE6 provides an experimental platform for studying the role of TMPRSS2 in viral entry into host cells.
The cell line can be used to construct disease models related to TMPRSS2, such as COVID-19 infection.
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