Human TMPRSS2 Stable Cell Line - HEK293T

Name: Human TMPRSS2 Stable Cell Line - HEK293T
SKU: CSC-RO01310
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO01310

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Cat. No.	CSC-RO01310
Description	HEK293T-Human-TMPRSS2 is engineered to stably overexpress human transmembrane serine protease 2 (TMPRSS2, NM_001135099.1). The overexpression of human TMPRSS2 has been validated by real-time qPCR and flow cytometry analysis.
Product Type	Human gene overexpression stable cell line
Target Gene	TMPRSS2
Gene Species	Homo sapiens (human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1) investigation of gene function 2) screening and validation of antibodies 4) anticancer drug development
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) Flow cytometry analysis of gene surface overexpression level 3) mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

TMPRSS2

Background

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2020]

CSC-RO01310-Human TMPRSS2 Stable Cell Line - HEK293T-Datasheet

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TMPRSS2 is a protein that belongs to the serine protease family. TMPRSS2 protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. TMPRSS2 gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of TMPRSS2 protein is thought to be cleaved and secreted into cell media after autocleavage. TMPRSS2 protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus).

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Customer Reviews

Great product!

The Human TMPRSS2 Stable Cell Line in HEK293T has been absolutely essential for our virology studies on viral entry mechanisms, particularly for SARS-CoV-2 research. The cells are stable, easy to maintain, and the expression persists through multiple passages. A must-have tool for anyone studying TMPRSS2-dependent viral infections or developing protease inhibitors.

United States

2024-09-27

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Human TMPRSS2 Stable Cell Line - HEK293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Background

Q & A

Customer Reviews

Great product!

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