Cat.No. : CSC-RO0692 Host Cell: HEK293T
| Cat. No. | CSC-RO0692 |
| Description | This cell line is engineered to stably overexpress human nectin cell adhesion molecule 4 (NECTIN4, LNIR, PRR4, EDSS1, PVRL4) in HEK293T. |
| Gene | NECTIN4 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The adoption of checkpoint inhibitors has transformed cancer treatment. However, these therapies frequently induce immune-related side effects, primarily attributed to their limited tumor specificity. Researchers investigated the role of NECTIN4 Stable Cell Line in cancer immunotherapy. They identified Nectin4 as a novel TIGIT ligand, crucial for inhibiting natural killer cell activity. By developing blocking Nectin4 antibodies, they demonstrated enhanced tumor killing in vitro and in vivo. This discovery suggests Nectin4-blocking antibodies could offer tumor specificity and immune checkpoint activity, potentially improving cancer immunotherapy safety and efficacy.
Figure 1. The function of NECTIN4 Stable Cell Line was explored by researchers. Nectin4 was cloned into lentiviral vectors containing GFP, allowing for its overexpression in cells. Checkpoint inhibitor antibodies targeting Nectin4 were tested for their ability to block TIGIT binding. Flow cytometry analysis revealed antibody binding to Nectin4-overexpressing cells and inhibition of TIGIT binding. Microscale thermophoresis was utilized to quantify the avidity between Nectin4 and anti-Nectin4 antibodies. (Reches A, et al., 2020)
A: MC38 cells were likely chosen for their relevance to cancer research and their ability to support the expression of CD19, facilitating the study of CD19 biology and its role in immune cell function and cancer immunotherapy.
A: Stability was likely assessed through methods such as flow cytometry, immunoblotting, or functional assays measuring CD19-mediated signaling, with continuous selection pressure applied.
A: Characterization may involve analysis of CD19 membrane localization, downstream signaling pathways, and functional implications in B cell activation, proliferation, and response to immunotherapeutic agents.
A: Quality control likely included confirmation of CD19 expression levels, validation of its functional activity, assessment of off-target effects, and validation of phenotypic changes associated with CD19 modulation.
A: Comparative analysis with primary immune cells or in vivo models helps validate the relevance of CD19 expression in B cell malignancies, immune evasion mechanisms, and its potential as a target for immunotherapy, guiding the development of CD19-targeted cancer treatments.
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Outstanding reliability! The Human NECTIN4 Stable Cell Line in HEK293T cells offers consistent NECTIN4 expression, ensuring dependable results in my cancer and virology research.
Enabling advanced investigations! With stable NECTIN4 expression, I can explore viral entry mechanisms and tumor progression pathways with confidence, advancing scientific understanding.
Impressive versatility! This cell line surpasses expectations, providing a reliable platform for studying NECTIN4-targeted therapies and viral oncolytic strategies.
Enhancing research efficiency! Its stable expression streamlines experimental workflows, simplifying data interpretation and accelerating scientific discoveries.
A pivotal resource! The Human NECTIN4 Stable Cell Line has significantly contributed to my research, offering valuable insights into NECTIN4-mediated viral infections and potential therapeutic interventions.
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