Cat.No. : CSC-RO0691 Host Cell: CHO-K1
| Cat. No. | CSC-RO0691 |
| Description | This cell line is engineered to stably overexpress human nectin cell adhesion molecule 4 (NECTIN4, LNIR, PRR4, EDSS1, PVRL4) in CHO-K1. |
| Gene | NECTIN4 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Nectin-4, also known as PVRL4 (poliovirus receptor-like 4), is specifically expressed in the embryo and placenta. Recent studies have reported that Nectin-4 is overexpressed in a variety of human cancers, and this aberrant expression is associated with cancer progression and poor patient prognosis. Here, researchers first examined the expression of Nectin-4 in human esophageal cancer (EC) tissues using immunohistochemistry (IHC) assays and analyzed the clinical relevance. In vitro cell studies and nude mouse in vivo tumor models were then used to examine the regulatory role of Nectin-4 in EC progression. The results showed that overexpression of Nectin-4 in human EC tissues was significantly associated with tumor size, tumor invasion depth, and poor patient prognosis. Intervention of Nectin-4 expression in EC cell lines showed that increased Nectin-4 expression could significantly promote cell viability, migration, invasion, and tumor formation. Therefore, Nectin-4 plays a crucial role in tumor biology and it can serve as a useful prognostic predictor for human EC.
Figure 1. Intervention of Nectin-4 expression affects the migration ability of human EC cell lines. The effect of intervention of Nectin-4 expression on the migration ability of human EC cell lines was evaluated by scratch healing assay. a The migration ability of Eca-109 and TE-1 cells was significantly reduced after knockdown Nectin-4 expression, and the cell-free area of the LV-Nectin-4-shRNA group was significantly larger than that of the LV-NC group at 24 h. b The migration ability of Eca-109 and TE-1 cells was significantly enhanced after overexpression of Nectin-4, and the cell-free area of the LV-Nectin-4-OE group was significantly smaller than that of the LV-Vector-Ctrl group at 24 h. (Deng H, et al. 2019)
Creative Biogene has successfully constructed NECTIN4 over-expressing stable cell lines with CHO-K1, HEK293, Eca-109, TE-1, CHO, HeLa, 1321N1, U2OS, RH7777, etc. These NECTIN4 stable cell lines have excellent in vitro assay sensitivity and reproducibility. Therefore, conducting experiments with our NECTIN4 stable cell lines saves time and resources while providing more accurate results.
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We are very pleased with the growth pattern and viability of the Human NECTIN4 Stable Cell Line - CHO-K1. The cells exhibit excellent proliferation rates and remain healthy even after multiple passages.
The stable expression of NECTIN4 in this CHO-K1 cell line has transformed our research. We have observed robust NECTIN4 protein production, which is critical for our binding assays and functional studies, ultimately accelerating our project timelines.
The quality of the Human NECTIN4 Stable Cell Line - CHO-K1 is matched by the excellent customer service provided. Any questions or concerns we had were addressed promptly and thoroughly.
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