Human MET Stable Cell Line - Ba/F3

Hepatocyte growth factor (HGF) activates the c-MET receptor, which is crucial for tissue regeneration and cellular processes like migration and proliferation. However, the clinical application of HGF is limited due to its instability and poor recombinant expression. The researchers engineered a more stable and expressible HGF fragment, eNK1, and developed a dimerized version of eNK1 that showed significantly improved c-MET activation and cellular responses compared to the monomer. In this study, the researchers used BaF3-MET cells, a murine cell line transfected with human c-MET receptors, to analyze the binding affinity and activity of the eNK1 monomer and dimer. They found that the eNK1 dimer had a much lower IC50, indicating stronger binding affinity. This research highlights the potential of Creative Biogene's Human MET Stable Cell Line - BaF3 as a tool for similar studies on c-MET receptor activation and its implications for regenerative medicine

Figure 1. The researchers treated HUVEC cells with HGF, eNK1 monomer, or eNK1 dimer at various concentrations to assess c-MET activation. They used Western blot analysis to detect phosphorylated c-MET, Akt, and Erk and employed the PathHunter system to quantify c-MET activation through chemiluminescence. BaF3-MET cells were used for binding assays to measure affinity. (Hassannia H, et al., 2022)