Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2575 Host Cell : HEK293
| Cat. No. | CLOE-2575 |
| Introduction | This cell lysate over-expresses mouse Met (NP_032617.2) (Met 1-Asn 929) fused with C-terminal his tag. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | Met |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Met met proto-oncogene [ Mus musculus ] |
| Gene Symbol | Met |
| Synonyms | HGF; HGFR; Par4; c-Met; AI838057 |
| Gene Description | met proto-oncogene |
| GeneID | 17295 |
| Uni ProtID | A4FUV6 |
| mRNA Refseq | NM_008591.2 |
| Protein Refseq | NP_032617.2 |
| Chromosome Location | 6 A2; 6 4.0 cM |
| Function | ATP binding; beta-catenin binding; hepatocyte growth factor-activated receptor activity; hepatocyte growth factor-activated receptor activity; kinase activity; nucleotide binding; phosphatidylinositol 3-kinase binding; phospholipase binding; protein complex binding; protein heterodimerization activity; protein kinase activity; protein tyrosine kinase activity; transferase activity; |
| Pathway | Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; |
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