Cat.No. : CSC-RO0672 Host Cell: HEK293
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0672 |
| Description | This cell line is engineered to stably overexpress human MER proto-oncogene, tyrosine kinase (MERTK) in HEK293. |
| Target Gene | MERTK |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Atherosclerosis is a complex vascular disease characterized by the accumulation of lipids and immune cells, leading to plaque formation in arterial walls. Research has identified the MERTK gene, which encodes a ligand-activated transmembrane tyrosine kinase, as a potential contributor to the disease process. Researchers investigated the influence of strain differences in MERTK on plaque formation by creating a congenic mouse line. This study revealed that mice with the DBA/2J allele of MERTK developed significantly larger atherosclerotic plaques compared to control mice. The findings highlighted that reduced expression of MERTK in macrophages from these mice correlated with impaired phagocytosis of apoptotic cells, shedding light on the role of MERTK in atherogenesis.
Figure 1. The researchers employed HEK293T cells to investigate MERTK expression and its impact on phagocytosis, aiming to understand its role in atherosclerosis. (Kayashima Y, et al., 2017)
Creative Biogene's Human MERTK Stable Cell Line - HEK293 is designed for researchers seeking to explore MERTK's functional role in various cellular processes, including apoptosis and immune responses. This cell line offers a reliable platform for studying the relationship between MERTK levels and phagocytic activity, facilitating the understanding of MERTK's influence on atherosclerosis and other diseases.
A: The overexpression of MERTK in this cell line provides a tool to study its involvement in efferocytosis, the process by which dead cells are cleared by phagocytes. Researchers can observe the engulfment process and downstream signaling pathways, shedding light on how defects in efferocytosis contribute to chronic inflammation.
A: Kinase assays using cell lysates or live cells can be employed to screen for inhibitors of MERTK kinase activity. Subsequent assays might include measuring changes in downstream signaling, cell proliferation, and apoptosis to assess the inhibitors' potency and selectivity.
A: Co-culture experiments with immune cells or other tumor cell lines can help to elucidate the cross-talk between MERTK and other cell surface receptors. Techniques such as immunoprecipitation and mass spectrometry could reveal interactions and signal integration relevant to tumorigenesis.
A: Wound healing assays and transwell migration assays can be conducted to examine how MERTK signaling affects the cell's motility and invasive potential. Such functional assays would clarify MERTK's role in cancer cell metastasis.
A: Although HEK293 cells are not macrophages, the stable expression of MERTK allows for the study of signaling pathways that influence macrophage-like behaviors. By mimicking the macrophage environment or by genetic manipulation, researchers can investigate how MERTK signaling affects behaviors associated with different macrophage polarization states.
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HEK293 cells harboring stable expression of Human MERTK ensure consistent and reliable levels of the receptor for downstream experiments.
Utilizing HEK293 cells as the host ensures efficient and stable expression of MERTK, minimizing the need for frequent cell passaging.
The stable MERTK expression in HEK293 cells allows for scalable production of cells for large-scale experiments or screening assays.
Stable MERTK expression in HEK293 cells leads to decreased experimental variability, enhancing the reliability and reproducibility of results.
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