Human MERTK Knockdown Cell Line - THP-1

1. Immunology and Cancer Research: Because it was derived from individuals with acute monocytic leukemia, the Human MERTK Knockdown Cell Line, or THP-1, is used in immunology and cancer research. When activated by phorbol esters, THP-1 cells can grow both adherently and in suspension, simulating macrophages and making research on immune responses and cancer progression easier. 2. Cell Physiology Studies: Using this cell line, researchers can investigate physiological processes such as cell survival, motility, differentiation, and phagocytosis of apoptotic cells (efferocytosis) that are regulated by MERTK. The research of MERTK's involvement in different cellular processes is made possible by the knockdown of its expression in these cells. 3. RPE Phagocytosis Pathway Investigation: Because the retinal pigment epithelium (RPE) phagocytosis pathway is disrupted, mutations in the MERTK gene are linked to autosomal recessive retinitis pigmentosa (RP). Through examining the consequences of MERTK knockdown in THP-1 cells, scientists can learn more about the molecular processes that underlie RP and possible targets for treatment. 4. Signaling Pathways Downstream: Autophosphorylation of MERTK following ligand binding sets off signaling cascades downstream that include molecules such as FAK/PTK2, RAC1, GRB2, PLCG2, MAPK1, and MAPK2. The investigation of these signaling pathways and their involvement in cellular processes and disease development is made possible by this cell line. 5. Drug Discovery and Development: By using this cell line to better understand the roles of MERTK and its signaling pathways in disease processes, targeted therapeutics for diseases like cancer and retinitis pigmentosa can be developed, advancing drug discovery efforts and personalized medicine techniques.