Cat.No. : CSC-SC004932-1 Host Cell: HEK293
| Cat. No. | CSC-SC004932-1 |
| Description | This cell line is engineered to stably express human endoglin (ENG) in HEK293 cells. |
| Gene | ENG |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Cluster of differentiation 105 (CD105), another name for endoglin (ENG), is an accessory receptor in the TGFβ signaling cascade. The breakdown mechanisms of the endoglin natural type and two mutant forms, P165L and V105D, linked to hereditary hemorrhagic telangiectasia type 1 (HHT1) were examined by the researchers using a Human ENG Stable Cell Line, HEK293. Protease and lysosomal inhibitors were applied to transfected HEK293 cells in a stable manner in an effort to uncover the molecular processes behind the loss of function in these variations. The results of the investigation showed that whereas the mutants primarily degrade via the proteasomal pathway, wild-type endoglin has a short half-life and degrades across both pathways. These findings point to possible treatment targets and implicate the ERAD process in HHT1 disease.
Figure 1. The researchers transfected HEK293 cells with HA-tagged WT Endoglin and mutant variants P165L and V105D using the pcDNA3.0 vector. They conducted a Cycloheximide chase assay to study protein stability. Results showed differential aggregation states among WT and mutant variants, analyzed by Western blotting and quantified relative to untreated controls, normalized against GAPDH. (Gariballa N, et al., 2022)
A: The Human ENG Stable Cell Line - HEK293 ensures stable expression of the ENG gene by introducing and integrating the ENG gene into HEK293 cells, utilizing selective antibiotic resistance markers and a screening process. This stable expression allows the cell line to continuously produce ENG protein, providing a reliable platform for studying endothelial cell functions and related diseases.
A: The application of the Human ENG Stable Cell Line - HEK293 in angiogenesis research includes modeling the behavior of vascular endothelial cells and studying how the ENG gene regulates vascular neovascularization and stability. This cell line can help scientists understand the molecular mechanisms of the angiogenesis process, providing a foundation for developing new therapies for vascular-related diseases.
A: The value of the Human ENG Stable Cell Line - HEK293 in studying the relationship between ENG gene mutations and diseases lies in its ability to simulate the effects of ENG gene mutations on cellular functions. With this cell line, scientists can study how ENG gene mutations lead to abnormal vascular development, which is crucial for understanding hereditary vascular diseases and developing targeted treatment strategies.
A: The role of the Human ENG Stable Cell Line - HEK293 in studying endothelial cell signaling is to simulate the role of the ENG gene within the cell, investigating how it affects cellular signaling pathways. This helps reveal how endothelial cells respond to external stimuli and how these signals regulate vascular function.
A: The importance of the Human ENG Stable Cell Line - HEK293 in studying vascular disease models lies in its ability to simulate the behavior of endothelial cells in disease states. This cell line allows scientists to study the role of the ENG gene in the development of vascular diseases, providing an experimental basis for developing new therapeutic targets and strategies.
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The Human ENG Stable Cell Line - HEK293 is optimized for high-level expression of the endoglin receptor (ENG), which is crucial for drug screening and functional studies.
Human ENG Stable Cell Line - HEK293 enables efficient drug testing, accelerating the evaluation of drug effects on ENG-related pathways.
Suitable for researching ENG as a potential biomarker, the Human ENG Stable Cell Line - HEK293 facilitates disease diagnostics and prognostic assessments.
As a powerful tool for studying the role of ENG in angiogenesis, Human ENG Stable Cell Line - HEK293 aids in the development of new therapies for vascular diseases.
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