Human EML4-ALK-D1203N-F1174C Stable Cell Line - Ba/F3

Name: Human EML4-ALK-D1203N-F1174C Stable Cell Line - Ba/F3
SKU: CSC-RO0326
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0326

Host Cell : Ba/F3 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0326
Description	This cell line is engineered to stably overexpress exogenous EML4-ALK fusion gene with F1174C and D1203N mutations in the ALK part.
Target Gene	EML4-ALK
Gene Species	Homo sapiens (Human)
Host Cell	Ba/F3
Host Cell Species	Mus musculus (Mouse)
Applications	Drug screening and biological assays
Biosafety Level	2
Size	>1x10^6 frozen cells/vial, 1 mL
Stability	Stable in culture over a minimum of 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Growth Conditions	37 °C, 5% CO₂
Storage	Liquid nitrogen
Shipping	Dry ice

Thawing & Subculturing Instructions	1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL.
Freeze Medium	Frozen with 70% medium, 20% FBS, 10% DMSO
Growth Properties	Suspension, round
Freezing Instructions	Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

EML4-ALK

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

The EML4-ALK-D1203N-F1174C gene mutation is a specific alteration that occurs in the EML4-ALK fusion gene, which is responsible for encoding the EML4-ALK fusion protein. This fusion protein is a tyrosine kinase that plays an essential role in cell growth, proliferation, and migration.Mutations in the EML4-ALK gene can lead to uncontrolled cell growth, commonly seen in non-small cell lung cancer (NSCLC), particularly in an isoform known as positive lymphoma kinase (ALK).

The EML4-ALK-D1203N-F1174C mutation is a complex mutation involving two specific alterations: D1203N and F1174C. The D1203N mutation occurs at amino acid 1203, resulting in the substitution of aspartic acid for asparagine, and the F1174C mutation occurs at amino acid 1174, resulting in the substitution of phenylalanine for cysteine. Together, these alterations affect the structure and function of the EML4-ALK fusion protein, leading to its constitutive activation and promoting cell growth, survival and migration.

This particular mutation is of interest in non-small cell lung cancer as it is often associated with a poor prognosis and limited treatment options.The EML4-ALK-D1203N-F1174C mutation can produce resistance to standard chemotherapeutic agents and alter the sensitivity of cancer cells to a variety of therapeutic approaches.

The human EML4-ALK-D1203N-F1174C stable cell line, BaF3, is an important tool for researchers to study the function and signalling pathway of the EML4-ALK fusion gene.The EML4-ALK fusion gene is produced by the fusion of the EML4 gene and the ALK gene, which results in the production of a fusion protein with constitutively active tyrosine kinase activity.

The D1203N and F1174C mutations in the ALK gene are known to produce resistance to ALK inhibitors, making this stable cell line an important model for studying the mechanisms of resistance and developing new therapies to overcome resistance.

This stable cell line can be used to test the efficacy of new ALK inhibitors and to study the signalling pathways downstream of mutant ALK proteins. It provides an important tool for the study and development of targeted therapies for NSCLC patients with mutations in the EML4-ALK fusion gene, including those resistant to existing inhibitors.

In conclusion, the human EML4-ALK-D1203N-F1174C stable cell line, BaF3, plays an important role in the study and development of new targeted therapies against this aggressive lung cancer.

Customer Q&As

What is the EML4-ALK-D1203N-F1174C gene and its relevance to cancer?

A: The EML4-ALK-D1203N-F1174C gene represents a specific variant of the EML4-ALK fusion gene, which is found in some cases of non-small cell lung cancer (NSCLC). The D1203N and F1174C mutations occur within the ALK (anaplastic lymphoma kinase) portion of the fusion protein, altering its structure and function. This variant is known to contribute to the development of aggressive lung cancer and is a target for targeted therapies.

How do the D1203N and F1174C mutations affect ALK function?

A: The D1203N and F1174C mutations in the EML4-ALK fusion protein affect ALK function by altering its kinase domain. These changes can lead to increased kinase activity, which in turn promotes uncontrolled cell growth and survival. This hyperactivity is a key driver of the oncogenic process in some NSCLC cases.

Are there any therapeutic implications of targeting the EML4-ALK-D1203N-F1174C fusion gene?

A: Targeting the EML4-ALK-D1203N-F1174C fusion gene has significant therapeutic implications. Inhibitors of ALK kinase activity, such as crizotinib (Xalkori) and newer ALK inhibitors, can be effective in treating NSCLC with this specific fusion variant. These targeted therapies can lead to tumor regression and improve patient outcomes.

How is the EML4-ALK-D1203N-F1174C fusion gene detected and diagnosed?

A: The EML4-ALK-D1203N-F1174C fusion gene is detected through molecular diagnostic techniques such as reverse transcription polymerase chain reaction (RT-PCR), fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS). These methods can identify the specific fusion event and the presence of the D1203N and F1174C mutations, allowing for accurate diagnosis and treatment planning for patients with NSCLC and this fusion variant.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

Literature Citation and Recognition

The Human ETV6-SYK Stable Cell Line - BaF3 has been widely cited in scientific literature, indicating its significant contribution to research on ETV6-SYK fusion kinase and its role in hematological malignancies.

United States

2020-12-23

Product Diversity

Offering a range of cell lines, the Human ETV6-SYK Stable Cell Line - BaF3 adds to the diversity of research tools available for studying hematological cancers, enabling researchers to explore various aspects of the disease.

United Kingdom

2023-11-19

Customization Services

Researchers appreciate the provider's willingness to offer customization services for the Human ETV6-SYK Stable Cell Line - BaF3, allowing for tailored experiments that meet specific research needs and objectives.

Canada

2022-10-27

Stringent Quality Control

The Human ETV6-SYK Stable Cell Line - BaF3 undergoes rigorous quality control measures, ensuring that researchers receive a reliable and high-quality product that consistently performs in experiments.

Canada

2020-09-13

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RO0452	Human ERBB2_A775-G776_ins_YVMA Stable Cell Line - MC38	Inquiry
CSC-RO0350	Human KIT-Del(V559V560) Stable Cell Line - Ba/F3	Inquiry
CSC-RO0746	Human NRAS-G12C Stable Cell Line - Ba/F3	Inquiry
CSC-RO0322	Human EGFR-Y764-V765_ins_HH Stable Cell Line - Ba/F3	Inquiry
CSC-RO0392	Human FLT3-D835F Stable Cell Line - Ba/F3	Inquiry
CSC-RO0673	Human MERTK Stable Cell Line - THP-1	Inquiry
CSC-RO0744	Human NRAS-Q61R Stable Cell Line - Ba/F3	Inquiry
CSC-RO0317	Human EGFR-K745-E746_ins_IPVAIK Stable Cell Line - Ba/F3	Inquiry
CSC-RO0344	Human ERBB2-G776VC Stable Cell Line - Ba/F3	Inquiry
CSC-RO0431	Human METex14Del Stable Cell Line - Ba/F3	Inquiry

Human EML4-ALK-D1203N-F1174C Stable Cell Line - Ba/F3

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the EML4-ALK-D1203N-F1174C gene and its relevance to cancer?

How do the D1203N and F1174C mutations affect ALK function?

Are there any therapeutic implications of targeting the EML4-ALK-D1203N-F1174C fusion gene?

How is the EML4-ALK-D1203N-F1174C fusion gene detected and diagnosed?

Literature Citation and Recognition

Product Diversity

Customization Services

Stringent Quality Control

Related Products

Related Services