Cat.No. : CSC-RO0710 Host Cell: HEK293
| Cat. No. | CSC-RO0710 |
| Description | This cell line is engineered to stably express human CRBN in HEK293. |
| Gene | CRBN |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
p53, among the most extensively studied tumor suppressors, plays a critical role in regulating apoptosis triggered by DNA damage. The researchers discovered that CRBN protects against DNA damage-induced apoptosis in cell lines and primary cells. Mechanistic studies reveal that CRBN interacts directly with p53, inhibiting its interaction with anti-apoptotic regulators Bcl-2 and Bcl-XL. This interaction preserves mitochondrial membrane potential and suppresses caspase-3 and PARP-1 cleavage, thereby inhibiting apoptosis in response to etoposide-induced DNA damage. Crbn knockout mice further validate these findings, highlighting CRBN's role in regulating DNA damage response and suggesting therapeutic implications for DNA damage-associated diseases.
Figure 1. The Human CRBN Stable Cell Line (HEK293) was created by the researchers to illustrate how CRBN interacts with p53. They used several techniques, including GST-tagged p53 incubation with His6-CRBN, co-transfection of GFP-CRBN and Flag-p53, and immunoprecipitation with anti-CRBN antibody. These methods clarified the relationship between CRBN's DNA-binding domain (DBD) and N terminus and p53. (Zhou L, et al., 2019)
A: This cell line, by stably expressing CRBN protein, can be used to study the role of CRBN in the cell cycle progression, especially in the regulatory mechanisms at the G1/S and G2/M checkpoints.
A: By stably expressing CRBN in HEK293 cells, researchers can model the expression pattern of CRBN in the nervous system and explore its function in neurodegenerative diseases such as Alzheimer's disease and potential therapeutic targets.
A: This cell line can be used for screening and evaluating drugs targeting CRBN, as abnormal expression of CRBN is associated with tumor growth and drug resistance in certain cancers.
A: This cell line can be used to study the regulatory role of CRBN when cells respond to stress conditions (such as oxidative stress, DNA damage), which is significant for understanding cellular stress responses and disease progression.
A: This cell line can be used to study the role of CRBN in cellular metabolic pathways, including the effects on glycolysis, fatty acid synthesis, and oxidative phosphorylation.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Human CRBN Stable Cell Line - HEK293 is specifically designed for efficient expression of the CRBN protein in HEK293 cells, which is crucial for studying the function of CRBN protein and drug screening.
By utilizing the Human CRBN Stable Cell Line - HEK293, researchers can quickly assess the effects of drugs on the CRBN protein, accelerating the discovery and development of new drugs.
This product ensures stable expression of the CRBN protein in HEK293 cells, which is key for conducting long-term experiments and reproducibility studies.
The Human CRBN Stable Cell Line - HEK293 is easy to culture and maintain under laboratory conditions, requiring no special culture conditions or complex operations, saving researchers time and resources.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
