Cat.No. : CSC-RO1119 Host Cell: A549
| Cat. No. | CSC-RO1119 |
| Description | This cell line is engineered to stably overexpress human CRBN in A549 cells. |
| Gene | CRBN |
| Gene Species | Homo sapiens (Human) |
| Host Cell | A549 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Especially in cancer therapy, cereblon (CRBN) is a crucial substrate receptor found in the Cullin 4-RING E3 ligase complex that regulates targeted protein degradation. Acting as a prophylactic agent against DNA damage-induced mortality, CRBN has been demonstrated to affect tumor suppressor p53. While CRBN does not alter the ubiquitination of p53, it interacts directly with it to stop its interaction with anti-apoptotic proteins such as Bcl-2 and Bcl-XL. This route improves cellular survival under DNA damage conditions, thereby underlining the therapeutic importance of CRBN in diseases linked to DNA damage response.
Figure 1. The researchers co-transfected HEK293 cells with GFP-p53 and mCherry-CRBN to investigate CRBN's influence on p53 subcellular distribution and protein levels using immunofluorescence and immunoblotting. (Zhou L, et al., 2019)
Creative Biogene's Human CRBN Stable Cell Line - A549 will provide researchers looking at the molecular involvement of CRBN in cancer biology a valuable source. Using this cell line, research on CRBN's interactions with p53 and its consequences on mortality may be undertaken, therefore directing therapeutic methods targeted at CRBN for cancer treatment.
A: The CRBN gene encodes for the cereblon protein, which is a component of the cullin-4 E3 ubiquitin ligase complex. Cereblon plays a critical role in the ubiquitination and subsequent degradation of target proteins, thereby regulating various cellular processes, including protein homeostasis and development.
A: The cereblon protein contributes to the regulation of protein levels through the ubiquitin-proteasome system. It binds to specific target proteins and facilitates their ubiquitination, which marks them for degradation by the proteasome. This process helps maintain protein balance and ensures the removal of misfolded or unwanted proteins.
A: Yes, the CRBN gene and its protein product have therapeutic implications, particularly in the treatment of certain cancers. Thalidomide and its derivatives, such as lenalidomide and pomalidomide, bind to cereblon and enhance its ability to ubiquitinate and degrade specific proteins involved in cancer cell proliferation and survival.
A: The CRBN gene influences the efficacy of thalidomide-based therapies by serving as a binding site for these drugs. The interaction between thalidomide derivatives and cereblon leads to the recruitment of target proteins, such as IKZF1 and IKZF3, for ubiquitination and degradation. This mechanism contributes to the antitumor effects of these therapies and can affect treatment response in patients.
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The Human CRBN Stable Cell Line - A549 is supported by comprehensive data and validation, including genetic sequencing and functional assays. This rigorous validation process ensures the reliability and accuracy of the experimental results and establishes the cell line as a reliable model for studying the functions and regulatory mechanisms of the CRBN gene.
The Human CRBN Stable Cell Line - A549 exhibits low immunogenicity and tumorigenicity, making it suitable for immune-deficient animal models and reducing potential risks associated with in vivo studies.
Stringent sterility and mycoplasma testing ensures the purity of the Human CRBN Stable Cell Line - A549, minimizing contamination concerns and providing researchers with a reliable cell line for experiments.
This cell line demonstrates high adaptability and transplantability, allowing for successful cultivation and functionality in various experimental settings, making it a versatile tool for research across different disciplines.
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