Human CRBN Stable Cell Line - SKOV3

Name: Human CRBN Stable Cell Line - SKOV3
SKU: CSC-RO0711
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0711

Host Cell : SKOV-3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0711
Description	This cell line is engineered to stably express human CRBN SKOV3.
Target Gene	CRBN
Gene Species	Homo sapiens (Human)
Host Cell	SKOV-3
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CRBN cereblon [ Homo sapiens ]
Gene Symbol	CRBN
Synonyms	CRBN; cereblon; mental retardation, non syndromic, autosomal recessive, 2A , MRT2A; protein cereblon; protein x 0001; MRT2; MRT2A; MGC27358; DKFZp781K0715;
Gene ID	51185
Uni Prot ID	Q96SW2
m RNA Refseq	BC017419
Chromosome Location	3p26.3
Function	ATP-dependent peptidase activity; protein binding;
MIM	609262

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Background

Applications

Q & A

Customer Reviews

The Human CRBN (Cereblon) gene encodes a protein that is part of the E3 ubiquitin ligase complex, which is involved in the degradation of specific target proteins through the ubiquitin-proteasome pathway. CRBN plays a crucial role in the regulation of various cellular processes, including cell cycle progression, apoptosis, and the immune response. Mutations or alterations in CRBN expression have been associated with several diseases, including certain types of cancer and neurological disorders.

The SKOV3 cell line, which originates from a human ovarian carcinoma, has been modified to stably express the Human CRBN gene. This stable cell line is instrumental for research into the functional consequences of CRBN expression and its role in cancer biology. It allows for the exploration of CRBN's interaction with other cellular components, its impact on cell survival and proliferation, and its potential as a therapeutic target. The SKOV3 CRBN stable cell line can also be used to evaluate the effects of drugs that target the CRBN pathway, contributing to the development of novel cancer treatments.

The Human CRBN Stable Cell Line - SKOV3 is a cell line derived from the ovarian cancer cell line SKOV3, engineered to express the CRBN gene. This cell line has several applications in cancer research:

(1)Therapeutic Targeting: CRBN is involved in the degradation of certain proteins, including those implicated in cancer. This cell line can be used to study the role of CRBN in cancer biology and to develop drugs that target the CRBN pathway.

(2)Drug Resistance: The CRBN gene has been implicated in the development of drug resistance in cancer cells. Researchers can use this cell line to investigate mechanisms of resistance and to identify strategies to overcome it.

(3)Protein-Protein Interactions: The stable expression of CRBN allows for the study of its interactions with other proteins, which can provide insights into the complex networks of signaling pathways in cancer cells.

Customer Q&As

How is the stable expression of CRBN protein achieved in the Human CRBN Stable Cell Line - SKOV3?

A: The Human CRBN Stable Cell Line - SKOV3 is created by introducing stable expression of the CRBN (Cereblon) gene into the SKOV3 ovarian cancer cell line. This is typically achieved by using viral vectors, such as retroviruses or lentiviruses, to insert the CRBN gene into the genome of SKOV3 cells, allowing for continuous expression of the CRBN protein and creating a platform for studying CRBN function and drug screening.

What is the role of the Human CRBN Stable Cell Line - SKOV3 in studying CRBN-mediated protein degradation?

A: The Human CRBN Stable Cell Line - SKOV3 provides a model system for studying CRBN-mediated protein degradation. CRBN is a component of the CRL4 E3 ubiquitin ligase complex, involved in the ubiquitination and subsequent proteasomal degradation of various substrate proteins. With this cell line, scientists can investigate how CRBN affects the degradation of specific substrate proteins, which is significant for understanding cellular signaling and disease therapy.

What is the application of the Human CRBN Stable Cell Line - SKOV3 in developing drugs targeting CRBN?

A: The Human CRBN Stable Cell Line - SKOV3 can be used to screen and evaluate drug candidates targeting CRBN. By monitoring CRBN-mediated protein degradation activity, researchers can identify small molecules that modulate CRBN activity, which is crucial for developing new drugs to treat diseases associated with CRBN.

What contribution does the Human CRBN Stable Cell Line - SKOV3 make in studying the relationship between CRBN and cancer development?

A: The Human CRBN Stable Cell Line - SKOV3 contributes to the study of CRBN's role in cancer development, particularly in ovarian cancer. CRBN plays a significant role in maintaining the cell cycle and inhibiting tumor formation. With this cell line, scientists can explore how CRBN affects the growth and survival of cancer cells and how inhibiting CRBN activity can suppress tumor development.

What is the role of the Human CRBN Stable Cell Line - SKOV3 in studying the role of CRBN in cellular stress responses?

A: The Human CRBN Stable Cell Line - SKOV3 contributes to the study of CRBN's role in cellular stress responses, particularly in dealing with DNA damage and oxidative stress. CRBN may be involved in regulating the degradation of stress-associated proteins, thereby affecting the cell's response to stress. With this cell line, scientists can better understand the function of CRBN in cellular stress responses, which is significant for developing therapeutic strategies against stress-related diseases.

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Customer Reviews

High Stability

The Human CRBN Stable Cell Line - SKOV3 offers a high degree of genetic stability, ensuring consistent and replicable expression of CRBN, which is crucial for the reliability of experimental outcomes.

French

2020-02-27

Facilitates Drug Screening

The Human CRBN Stable Cell Line - SKOV3, exhibiting excellent CRBN protein activity, is highly suitable for the discovery and testing of new pharmaceuticals.

United States

2020-03-29

Disease Model Research

Serving as a disease model for human ovarian cancer, the Human CRBN Stable Cell Line - SKOV3 provides an effective tool for studying the role of CRBN in tumor progression.

French

2022-06-14

Protein Interaction Research

Utilizing the Human CRBN Stable Cell Line - SKOV3 can facilitate the study of interactions between CRBN protein and other molecules, advancing molecular-level understanding.

Canada

2020-02-24

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Human CRBN Stable Cell Line - SKOV3

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

How is the stable expression of CRBN protein achieved in the Human CRBN Stable Cell Line - SKOV3?

What is the role of the Human CRBN Stable Cell Line - SKOV3 in studying CRBN-mediated protein degradation?

What is the application of the Human CRBN Stable Cell Line - SKOV3 in developing drugs targeting CRBN?

What contribution does the Human CRBN Stable Cell Line - SKOV3 make in studying the relationship between CRBN and cancer development?

What is the role of the Human CRBN Stable Cell Line - SKOV3 in studying the role of CRBN in cellular stress responses?

High Stability

Facilitates Drug Screening

Disease Model Research

Protein Interaction Research

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