Cat.No. : CSC-RO0711 Host Cell: HEK293
| Cat. No. | CSC-RO0711 |
| Description | This cell line is engineered to stably express human CRBN SKOV3. |
| Gene | CRBN |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CRBN Stable Cell Line - SKOV3 is created by introducing stable expression of the CRBN (Cereblon) gene into the SKOV3 ovarian cancer cell line. This is typically achieved by using viral vectors, such as retroviruses or lentiviruses, to insert the CRBN gene into the genome of SKOV3 cells, allowing for continuous expression of the CRBN protein and creating a platform for studying CRBN function and drug screening.
A: The Human CRBN Stable Cell Line - SKOV3 provides a model system for studying CRBN-mediated protein degradation. CRBN is a component of the CRL4 E3 ubiquitin ligase complex, involved in the ubiquitination and subsequent proteasomal degradation of various substrate proteins. With this cell line, scientists can investigate how CRBN affects the degradation of specific substrate proteins, which is significant for understanding cellular signaling and disease therapy.
A: The Human CRBN Stable Cell Line - SKOV3 can be used to screen and evaluate drug candidates targeting CRBN. By monitoring CRBN-mediated protein degradation activity, researchers can identify small molecules that modulate CRBN activity, which is crucial for developing new drugs to treat diseases associated with CRBN.
A: The Human CRBN Stable Cell Line - SKOV3 contributes to the study of CRBN's role in cancer development, particularly in ovarian cancer. CRBN plays a significant role in maintaining the cell cycle and inhibiting tumor formation. With this cell line, scientists can explore how CRBN affects the growth and survival of cancer cells and how inhibiting CRBN activity can suppress tumor development.
A: The Human CRBN Stable Cell Line - SKOV3 contributes to the study of CRBN's role in cellular stress responses, particularly in dealing with DNA damage and oxidative stress. CRBN may be involved in regulating the degradation of stress-associated proteins, thereby affecting the cell's response to stress. With this cell line, scientists can better understand the function of CRBN in cellular stress responses, which is significant for developing therapeutic strategies against stress-related diseases.
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The Human CRBN Stable Cell Line - SKOV3 offers a high degree of genetic stability, ensuring consistent and replicable expression of CRBN, which is crucial for the reliability of experimental outcomes.
The Human CRBN Stable Cell Line - SKOV3, exhibiting excellent CRBN protein activity, is highly suitable for the discovery and testing of new pharmaceuticals.
Serving as a disease model for human ovarian cancer, the Human CRBN Stable Cell Line - SKOV3 provides an effective tool for studying the role of CRBN in tumor progression.
Utilizing the Human CRBN Stable Cell Line - SKOV3 can facilitate the study of interactions between CRBN protein and other molecules, advancing molecular-level understanding.
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