Cat.No. : CSC-RT1891
Host Cell: Hela Target Gene: TFRC
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT1891 |
| Description | This cell line is a stable cell line with a homozygous knockout of human TFRC using CRISPR/Cas9. |
| Target Gene | TFRC |
| Gene ID | 7037 |
| Genotype | TFRC (-/-) |
| Host Cell | Hela |
| Host Cell Species | Homo sapiens (Human) |
| Cell Type | Epithelial |
| Size | 1x10^6 cells/vial, 1mL |
| SequencingResult | Homozygous: 1 bp insertion in exon 4 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers evaluated DYNE-101, a FORCE conjugate targeting DMPK RNA to correct spliceopathy in myotonic dystrophy type 1 (DM1). Using patient-derived myoblasts, HSALR mice, TfR1hu/mu; DMSXLTg/Tg mice, and cynomolgus monkeys, they demonstrated that DYNE-101 reduces mutant DMPK RNA and nuclear RNA foci, restores normal splicing patterns, and alleviates myotonia. Cellular internalization studies using TfR1-deficient and wild-type HeLa cells confirmed that delivery depends on the transferrin receptor, while cardiomyocyte experiments showed efficient uptake and colocalization with early endosomes. These results validate FORCE as an effective delivery platform for low-dose, infrequent administration of oligonucleotide therapeutics in DM1.
Figure 1. Fluorescence imaging and flow cytometry showed that DYNE-101 or labeled FAB02 efficiently internalizes into TfR1-expressing cells and cardiomyocytes, while uptake is markedly reduced in TfR1−/− HeLa cells. (Weeden T, et al., 2025)
Creative Biogene's TfR1−/− HeLa cells enable precise assessment of receptor-mediated oligonucleotide internalization, supporting the evaluation and optimization of muscle-targeted conjugates like DYNE-101 for neuromuscular disease research.
A: DMEM supplemented with 10% fetal bovine serum.
It is not required to add the selection antibiotics when culturing the KO cells.
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The TFRC knockout cell model can be used extensively to study the role of TfR in various biological processes such as iron metabolism, cellular signaling, and proliferation. The cell line is very helpful for our research.
I use TFRC knockout cells to study the role of TFRC in cancer cell growth and survival. Good experimental results were obtained.
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