Cat.No. : CSC-RO1138 Host Cell: HEK293T
| Cat. No. | CSC-RO1138 |
| Description | This cell line is engineered to stably overexpress exogenous human transferrin receptor (TFRC) in HEK293T. |
| Gene | TFRC |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Iron-dependent ferroptosis causes lipid peroxidation and cell death. Hepatocellular carcinoma (HCC) overproduces the transferrin receptor (TFRC), which is essential for ferroptosis. The post-translational modifications (PTMs) of TFRC—especially O-GlcNAcylation—which influences hepatocyte susceptibility to Erastin-induced ferroptosis were examined by the researchers. Erastin they found causes de-O-GlcNAcylation at serine 687, which lowers ubiquitin E3 ligase MARCH8's binding capacity. This process decreases polyubiquitination at lysine 665, enhancing the stability of TFRC and promoting the accumulation of labile iron. These findings shed light on the intricate regulatory mechanisms of ferroptosis in HCC and highlight the significance of iron metabolism pathways.
Figure 1. The researchers transfected HEK293T cells with pcDNA3.1, pcDNA3.1-TFRC, and pcDNA3.1-OGA to evaluate how global O-GlcNAcylation alterations influence TFRC protein levels, revealing increased TFRC expression following co-transfection. (Zhou X, et al., 2024)
Creative Biogene's Human TFRC Stable Cell Line - HEK293T can serve as a valuable tool for further investigations into ferroptosis and related pathways in cancer research.
A: The TFRC gene encodes for the transferrin receptor 1 (TfR1), which is a cell surface protein that binds transferrin (Tf), the primary iron-carrying protein in the blood. This receptor is essential for the cellular uptake of iron, a vital nutrient for many metabolic processes. By mediating the endocytosis of Tf, TfR1 plays a key role in iron homeostasis and cell growth.
A: The TFRC gene contributes to iron homeostasis and cell growth by ensuring the efficient uptake of iron into cells. TfR1 facilitates the internalization of iron-loaded Tf, which is then released into the cytoplasm. This process is crucial for the production of hemoglobin in red blood cells and for the synthesis of essential proteins and enzymes in other cell types.
A: Mutations in the TFRC gene can lead to disorders affecting iron homeostasis and cellular iron utilization. These include hereditary hemochromatosis type 1, a condition characterized by excessive iron accumulation, and anemia of inflammation, where iron absorption is impaired. These mutations can alter the function of TfR1, leading to impaired iron regulation and cellular metabolism.
A: The expression of the TFRC gene is regulated by various factors, including iron availability and cellular demand. Iron deficiency can lead to increased expression of TfR1 to enhance iron uptake. Conversely, iron excess can downregulate TfR1 expression to prevent further iron accumulation. Transcription factors, such as IRP1 and IRP2, also play a role in regulating TFRC expression in response to iron levels.
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The Human TFRC Stable Cell Line - HEK293T is derived from a well-characterized human cell line, addressing ethical concerns associated with animal testing. However, proper safety protocols should be followed to handle the cell line, ensuring researcher safety and preventing unintended consequences.
The use of a stable cell line like Human TFRC Stable Cell Line - HEK293T can contribute to sustainable research practices. By providing a renewable source of cells for experimentation, it helps reduce the environmental impact associated with continuous animal sourcing and tissue harvesting.
The Human TFRC Stable Cell Line - HEK293T is made accessible to researchers worldwide, enabling collaboration and reproducibility of research findings across different geographical locations. This global availability facilitates the advancement of scientific knowledge and promotes international research partnerships.
The Human TFRC Stable Cell Line - HEK293T undergoes rigorous functional validation, including assays to confirm the expression and activity of TFRC. Researchers can trust the reliability and consistency of the cell line for their experiments, allowing for accurate interpretation of results and meaningful data analysis.
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