Human TFRC Knockout Cell Line-A549

Name: Human TFRC Knockout Cell Line-A549
SKU: CSC-RT2828
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2828

Target Gene : TFRC Host Cell : A549

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2828
Description	This cell is a stable cell line with a homozygous knockout of human TFRC using CRISPR/Cas9.
Target Gene	TFRC
Host Cell	A549
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TFRC transferrin receptor (p90, CD71) [ Homo sapiens ]
Gene Symbol	TFRC
Synonyms	TFRC; transferrin receptor (p90, CD71); transferrin receptor protein 1; CD71; TFR1; T9; TR; TFR; p90; TRFR;
GeneID	7037
Uni ProtID	P02786
mRNA Refseq	BC001188
Chromosome Location	3q26.2-qter
Function	Hsp70 protein binding; chaperone binding; peptidase activity; receptor activity; transferrin receptor activity;
Pathway	Clathrin derived vesicle budding, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem;
MIM	190010

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