Cat.No. : CSC-DC016549
Host Cell: HEK293 (Hela and other cell types are also available) Validation: Real-Time RCR
| Cat. No. | CSC-DC016549 |
| Description | Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free. |
| Gene | TREM2 |
| Host Cell | HEK293 (Hela and other cell types are also available) |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | (1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | >1 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid Nitrogen |
| Gene Name | TREM2 triggering receptor expressed on myeloid cells 2 [ Homo sapiens ] |
| Gene Symbol | TREM2 |
| Synonyms | TREM-2; Trem2a; Trem2b; Trem2c |
| Gene ID | 54209 |
| UniProt ID | Q5TCX1 |
| mRNA Refseq | NM_018965.2 |
| Protein Refseq | NP_061838.1 |
| Chromosome Location | 6p21.1 |
| Function | receptor activity; |
| Pathway | Axon guidance, organism-specific biosystem; DAP12 interactions, organism-specific biosystem; DAP12 signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; |
| MIM | 605086 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Renal cell carcinoma (RCC) is a common malignant tumor with poor prognosis and high mortality. The role of triggering receptor expressed on myeloid cells‑2 (TREM2) in RCC progression has been increasingly recognized, but its underlying mechanisms remain to be fully elucidated. Here, researchers evaluated the effects of TREM2 on RCC cells and its potential mechanisms. Results showed that TREM2 expression levels were significantly increased in cancerous tissues compared to adjacent normal tissues. CCK-8 and EdU assays indicated that TREM2 knockdown significantly inhibited RCC cell proliferation, while TREM2 overexpression promoted RCC cell proliferation. Scratch and Transwell assays showed that, compared to controls, TREM2 overexpression significantly enhanced RCC cell migration and invasion, while TREM2 knockdown significantly reduced RCC cell migration. Furthermore, Western blot analysis revealed that TREM2 knockdown in RCC cells significantly increased the phosphorylation levels of p53 and p21 proteins. In conclusion, TREM2 is highly expressed in RCC tissues and promotes RCC cell migration by inhibiting the p53 signaling pathway.
To assess the effect of TREM2 on renal cell carcinoma (RCC) cell migration, researchers conducted a scratch healing assay, observing wound healing at 0 and 24 hours. Compared to the sh-NC group, wound healing in the TREM2 knockdown ACHN cell line was significantly inhibited. Conversely, overexpression of TREM2 promoted wound healing in ACHN cells compared to the vector group (Figure 1A). These results indicate that TREM2 can enhance RCC cell migration. Transwell assays further showed that TREM2 knockdown significantly inhibited cell invasion compared to the sh-NC group, while overexpression of TREM2 significantly enhanced cell invasion compared to the vector group (Figure 1B). In summary, these results indicate that TREM2 promotes RCC cell migration and invasion.
Figure 1. TREM2 promotes migration of RCC cells. (Zhang L, et al., 2024)
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
